Synthesis of [7-¹⁵N]-GTPs for RNA structure and dynamics by NMR spectroscopy.

IF 1.7 Q3 ENGINEERING, GEOLOGICAL

Geomechanics and Geoengineering-An International Journal Pub Date : 2022-01-01 Epub Date: 2022-02-26 DOI:10.1007/s00706-022-02892-1

Kehinde M Taiwo, Lukasz T Olenginski, Felix Nußbaumer, Hyeyeon Nam, Stefan Hilber, Christoph Kreutz, T Kwaku Dayie

引用次数: 0

Abstract

Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-¹⁵N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s00706-022-02892-1.

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合成[7-15N]-GTPs，利用核磁共振光谱分析 RNA 结构和动力学。

为利用核磁共振 (NMR) 光谱研究 RNA，已经开发出多种同位素标记策略。在此，我们报告了[7-15N]-鸟苷-5'-三磷酸的化学和酶法联合合成方法，该方法可通过基于 T7 RNA 聚合酶的体外转录掺入 RNA 中。我们展示了这些标签在探测两种具有重要生物学意义的 RNA 的结构和动力学方面的用途：在线版本包含补充材料，可查阅 10.1007/s00706-022-02892-1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Geomechanics and Geoengineering-An International Journal ENGINEERING, GEOLOGICAL-

CiteScore

3.80

自引率

0.00%

发文量

期刊介绍： Geomechanics is concerned with the application of the principle of mechanics to earth-materials (namely geo-material). Geoengineering covers a wide range of engineering disciplines related to geo-materials, such as foundation engineering, slope engineering, tunnelling, rock engineering, engineering geology and geo-environmental engineering. Geomechanics and Geoengineering is a major publication channel for research in the areas of soil and rock mechanics, geotechnical and geological engineering, engineering geology, geo-environmental engineering and all geo-material related engineering and science disciplines. The Journal provides an international forum for the exchange of innovative ideas, especially between researchers in Asia and the rest of the world.

Synthesis of [7-15N]-GTPs for RNA structure and dynamics by NMR spectroscopy.

Abstract

Synthesis of [7-¹⁵N]-GTPs for RNA structure and dynamics by NMR spectroscopy.