Effect of methanolic extracts of Tectona grandis linn leaves on diabetic neuropathy in streptozotocin‒induced diabetic rats

Abstract

Tectona grandis TG plant belonging to family Verbenaceae it is medicinally reported and claims to cure various diseases in Indian traditional system Ayurveda and in folklore The purpose of this study is to examine the methanol extract of Tectona grandis leaves TGME in Streptozotocin STZ induced diabetic neuropathy in rat Initially acute oral toxicity study of TGME was carried out in rats to evaluate the dose for animal study STZ mg kg was used to induce diabetes in rats TGME was given orally dose of and mg kg to normal and diabetic rats from initiate th to th weeks total days Blood collection for estimation of glucose level biochemical parameters and behavioural parameters were assessed initially up to th week every nd week interval At the end of study after th week rats were sacrificed for estimation brain tissue estimations and histopathology of rat paw skin was also carried out In acute oral toxicity study result TGME do not cause any toxicity or death in animals Diabetic rat rsquo s significant increase in serum glucose and other biochemical parameters as well as mechanical allodynia was indicates hyperalgesia after weeks of diabetes induction Brain tissue estimations revealed increase in lipid peroxidation LPO level a decrease in antioxidant parameters and histopathology study of paw skins of rat rsquo s intraepidermal nerve fiber density was decreased in diabetic neuropathy STZ treated group Administration of dose of and mg kg of TGME significantly P lt decreased blood glucose level in normal and diabetic rats A dose of mg kg significantly P lt and P lt increases the tail withdrawal latency with hot and cold water tail immersion tests respectively Both the doses of TGME significantly increase the level of glutathione GSH and lower the levels of LPO catalase CAT and superoxide dismutase SOD Histopathology study of paw skin of rat intraepidermal nerve fiber density was increased as a result of drug administration The results concluded that TGME possesses potential to combat glucose hypolipidemic effect and scavenge free radicals in diseases associated with oxidative stress suggests its role in preventing diabetes related complications