Detection of gyrA and parC Genes in Clinical Acinetobacter Baumannii Isolates

Abstract

100 isolates of Acinetobacter baumannii were collected from different clinical sources including (blood, sputum and burns) from hospitals in Baghdad - Iraq. In order to investigate its resistance to fluoroquinolones. MIC assay for ciprofloxacin was performed using E-test, and PCR assay for gry A and parC genes. The results of the MIC showed that A. baumannii was sensitive to ciprofloxacin at concentration=<4 μg/ml. As for the PCR assay, the prevalence of gyr A gene in 40 of the isolates was 40%, while the par C gene in 16 of the isolates was 16%. This research shed light on the rapid spread of fluoroquinolone resistance that included both ciprofloxacin and levofloxacin among A.baumannii bacteria isolated from clinical sources.