Highly Sensitive Voltammetric Catechol Biosensor Based on Electroaddressing of Laccase Encapsulated in Modified Chitosan

Sensor Letters Pub Date : 2020-03-01 DOI:10.1166/sl.2020.4210

H. Galai, N. Rahmouni, P. Namour, A. Bonhomme, F. Bessueille, S. Hentati, N. Jaffrezic‐Renault

引用次数: 3

Abstract

In this work, a new voltammetric biosensor, based on the encapsulation of laccase from Trametes versicolor in an electrodeposited nanocomposite film, was developed for the detection of catechol. The nanocomposite was composed of chitosan (CS) modified with trymiristine (Tr), both of natural origin, to enhance its conductivity. FTIR and SEM studies were performed to characterize the modified chitosan film and the encapsulation of the enzyme. The analytical performance of the elaborated biosensor was determined through voltammetry detection of the produced quinone. Catechol was detected in a linear range between 10–20 and 10–15 M and a sensitivity of 0.4667 mA/p [catechol] was found. The repeatability of this biosensor is good as its RSD is equal to 1.35% and it shows stability for four weeks. To test the functionality of the developed biosensor, the total phenolic content of three samples of natural oils was determined and compared to a colorimetric test.

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改性壳聚糖包封漆酶电寻址的高灵敏度伏安式儿茶酚生物传感器

在本工作中，基于电沉积纳米复合膜中漆酶的包封，开发了一种新的伏安生物传感器，用于检测儿茶酚。该纳米复合材料由天然来源的trymiristine (Tr)修饰的壳聚糖(CS)组成，以提高其导电性。利用红外光谱(FTIR)和扫描电镜(SEM)对改性壳聚糖膜及其酶包封进行了表征。利用伏安法对合成的醌进行检测，确定了该传感器的分析性能。在10-20 ~ 10-15 M的线性范围内检测到儿茶酚，灵敏度为0.4667 mA/p[儿茶酚]。该生物传感器重复性好，RSD为1.35%，稳定性为4周。为了测试所开发的生物传感器的功能，测定了三种天然油样品的总酚含量，并与比色试验进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensor Letters 工程技术-电化学

自引率

0.00%

发文量

审稿时长

6 months

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