Relationship between PAK6 expression and radiosensitivity in breast cancer patients undergoing radiotherapy

Central Plains Medical Journal Pub Date : 2020-02-10 DOI:10.3760/CMA.J.ISSN.1674-4756.2020.03.006

Xiaohong Wang, Zhenkun Han, Jingjing Wang

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Abstract

Objective To investigate the relationship between P21-activated kinase 6 (PAK6) expression and radiosensitivity of breast cancer patients undergoing radiotherapy. Methods MDA-MB-453 breast cancer cell line, small interfering RNA (siRNA), and cells transfected with PAK6 siRNA were set as untransfected group, control group and observation group, respectively. Western blot and quantitative real time polymerase chain reaction were used to detect the expression of PAK6 in each group. Every group was given 0, 1, 3 and 5 Gy radiotherapies, respectively, with one radiation dose for once. Thereafter, cell proliferation was detected by cell counting kit-8, and apoptosis was monitored by flow cytometry. Western blotting was used to detect the level of Caspase-3 protein in cells of three groups. Results The PAK6 protein and PAK6 mRNA expression levels of the untransfected group and control group were higher than those of the observation group (P<0.05). The proliferation of breast cancer cells , with the radiotherapy doses of 0, 1, 3 and 5 Gy, in the untransfected group and the control group were higher than that in the observation group (P<0.05); while untransfected group and control group had lower levels of apoptosis than observation group (P<0.05). The expression level of caspase-3 in the observation group was higher than that in the control group and untransfected group (P<0.05). Compared with the untransfected group and control group, the cell inventory score of the observation group was reduced, and the sensitization ratio of radiotherapy was 1.774. Conclusions Interference on PAK6 expression in breast cancer cells can inhibit the proliferation of breast cancer cells, promote the apoptosis of breast cancer cells and increase the radiosensitivity of breast cancer patients. The mechanism may be related to the effects of PAK6 on caspase-3 protein. Key words: Breast cancer; P21-activated kinase 6; Radiotherapy; Sensitivity

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乳腺癌放疗患者PAK6表达与放射敏感性的关系

目的探讨乳腺癌放疗患者p21活化激酶6 (PAK6)表达与放射敏感性的关系。方法将MDA-MB-453乳腺癌细胞系、小干扰RNA (small interfering RNA, siRNA)和转染PAK6 siRNA的细胞分别作为未转染组、对照组和观察组。采用Western blot和实时定量聚合酶链反应检测各组中PAK6的表达。各组分别给予0、1、3、5 Gy放疗，每次放疗一次。用细胞计数试剂盒-8检测细胞增殖，流式细胞术检测细胞凋亡。Western blotting检测三组细胞中Caspase-3蛋白水平。结果未转染组和对照组的PAK6蛋白及PAK6 mRNA表达量均高于观察组(P<0.05)。放疗剂量为0、1、3、5 Gy时，未转染组和对照组乳腺癌细胞增殖均高于观察组(P<0.05);未转染组和对照组细胞凋亡水平低于观察组(P<0.05)。观察组caspase-3表达水平高于对照组和未转染组(P<0.05)。与未转染组和对照组比较，观察组细胞清查评分降低，放疗致敏比为1.774。结论干扰乳腺癌细胞中PAK6的表达可抑制乳腺癌细胞的增殖，促进乳腺癌细胞凋亡，增加乳腺癌患者的放射敏感性。其机制可能与PAK6对caspase-3蛋白的影响有关。关键词:乳腺癌;p21活化激酶6;放射治疗;灵敏度

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Central Plains Medical Journal

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