Методы рентгеновского флуоресцентного микроанализа и спектроскопии комбинационного рассеяния света для исследования гистологических срезов мышечных тканей

Журнал технической физики Pub Date : 2023-01-01 DOI:10.21883/jtf.2023.07.55752.75-23

И. А. Артюков, Григорий Павлович Арутюнов, Д. О. Драгунов, Н. Н. Мельник, Д.Х.А. Панэке, Е.В. Переведенцева, А. В. Соколова, В. В. Соколова

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Abstract

The paper presents the results of a study of the chemical composition of myocardial tissues and striated muscles at the cellular level using a combination of X-ray fluorescence microscopy and Raman microspectrosopy. The distribution of inorganic materials (Na, Si, Ca, etc.) was measured with the help of X-ray fluorescence imaging and microanalysis workstations at ELETTRA synchrotron (Italy). Methods of infrared Raman spectromicroscopy were applied for the identification of organic compounds (lipids, amides, and polysaccharides). All experiments were carried out with unstained paraffin-embedded histological sections. The research was related to the study of sodium deposits in muscle tissues.

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x射线荧光显微分析和光谱学光组合散射方法用于研究肌肉组织学剪切

本文介绍了利用x射线荧光显微镜和拉曼显微光谱技术在细胞水平上对心肌组织和横纹肌的化学成分进行研究的结果。借助意大利ELETTRA同步加速器的x射线荧光成像和微量分析工作站，测量了无机材料(Na, Si, Ca等)的分布。采用红外拉曼光谱显微镜对有机化合物(脂类、酰胺类、多糖类)进行鉴定。所有实验均采用未染色的石蜡包埋组织切片进行。这项研究与研究肌肉组织中的钠沉积有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Журнал технической физики

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