Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets

2019 IEEE International Ultrasonics Symposium (IUS) Pub Date : 2019-10-01 DOI:10.1109/ULTSYM.2019.8926069

Ge Zhang, P. Weinberg, C. Dunsby, M. Tang, S. Harput, M. Toulemonde, Jacob Broughton-Venner, Jiaqi Zhu, K. Riemer, K. Christensen-Jeffries, Jemma Brown, R. Eckersley

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引用次数: 6

Abstract

Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 µm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging.

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声波稀疏激活定位显微镜(AWSALM):利用纳米液滴在体内快速超声超分辨率成像

目前基于定位的超分辨率超声成像需要低浓度的流动微泡来显示超过衍射极限的微血管，并且采集速度很慢。纳米液滴提供了一个很有前途的解决方案，因为它们可以按需稀疏激活和停用。在这项研究中，声波稀疏激活定位显微镜(AWSALM)使用纳米液滴的激活和失活，这是光激活定位显微镜(PALM)的声学对应物，较少依赖于药物浓度和流动的存在，被证明可以在体内深层组织中进行超分辨率成像。使用AWSALM在1.1秒内获得了兔肾脏的体内超分辨率图像，其中显示了表观尺寸远低于220µm半波长的微血管。这一初步结果证明了将AWSALM应用于体内超分辨率成像的可行性。

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2019 IEEE International Ultrasonics Symposium (IUS)

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