Evaluation of CXCR4, VLA4, and VLA5 expression in peripheral mobilized hematopoietic stem cells in presence of Sotalol

Abstract

Hematopoietic stem cells (HSC), are considered as an important source for HSCT transplantation. There are several regulators for stem cells migration and homing, among which the nervous system is an important one. This study is going to investigate the   role of nervous system in homing of hematopoietic stem cells. In an experimental study, mononuclear cells were isolated from peripheral blood and treated by a beta adrenergic receptors blocker (Sotalol). After treating the cells with different concentrations 0.5- 500 (mM/ml) for 1- 24 hours, RNA was extracted and the expression of VLA4, VLA5 and CXCR4 genes were determinated through RT-PCR method.The results of this study demonstrated high expression in genes associated with homing of the cells after being treated with drug for 1 hour: CXCR4 gene expression increases in 10 and 50 mM/ml, VLA4 gene expression in 50mM/ml and VLA5 gene expression in 10 mM/ml concentration of Sotalol.Using beta adrenergic receptors blockers in appropriate time and dose of drug can affect the expression of genes which involved in HSCs homing and will lead to high success rate of transplantation of these cells.