Ahsan Aslam, Muhammad Saleh, Farzana Rahim Memon, Sadia Khan, Soobia Pathan, Kazbano Ramsha
{"title":"Histomorphometric effects of Raphanus Sativus leaf extract on Carbon Tetrachloride induced hepatotoxicity","authors":"Ahsan Aslam, Muhammad Saleh, Farzana Rahim Memon, Sadia Khan, Soobia Pathan, Kazbano Ramsha","doi":"10.35787/jimdc.v11i1.684","DOIUrl":null,"url":null,"abstract":"Background: Raphanus sativus is a cruciferous plant which possess free radical scavenging properties that defend the body against the deleterious effects of reactive oxygen species. To evaluate the protective role of Raphanus sativus in CCl4-induced hepatotoxicity through assessment of liver function and oxidative stress markers in albino Wistar rats. \nMaterial and Methods: Quasi-experimental study was conducted from September 2019 to March 2020 at the Postgraduate Research Laboratory, Isra University, Hyderabad. Non-random purposive sampling was used for selecting Albino Wistar rats. All rats were then divided equally into three different groups: Group A (control group), Group B (CCl4 experimental group), and Group C (CCl4 plus Raphanus sativus group). Blood samples were collected through cardiac puncture followed by hepatic histopathological analysis using light microscope. Data was analyzed using SPSS version 24, with ANOVA and Post hoc Tukey analysis used for comparison of different study variables. \nResults: Statistically significant rise relative liver weight of group B rats compared with the group A and C rats (P <.05). Moreover, statistically significant (P <0.05) rise in serum markers of hepatic functions was observed after CCl4 administration in Group B. Treatment with Raphanus Sativus administration significantly reduced serum levels of LFTs (p<0.05). There was a significant decline in the plasma levels of oxidative markers in group B while marked histopathological changes like necrosis, sinusoidal dilatation and congestion observed among animals of group B. \nConclusion: Raphanus Sativus exerts an anti-oxidative, anti- and hepatoprotective effect against CCl4-induced hepatic tissue damage.","PeriodicalId":33701,"journal":{"name":"Journal of Islamabad Medical and Dental College","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Islamabad Medical and Dental College","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35787/jimdc.v11i1.684","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Raphanus sativus is a cruciferous plant which possess free radical scavenging properties that defend the body against the deleterious effects of reactive oxygen species. To evaluate the protective role of Raphanus sativus in CCl4-induced hepatotoxicity through assessment of liver function and oxidative stress markers in albino Wistar rats.
Material and Methods: Quasi-experimental study was conducted from September 2019 to March 2020 at the Postgraduate Research Laboratory, Isra University, Hyderabad. Non-random purposive sampling was used for selecting Albino Wistar rats. All rats were then divided equally into three different groups: Group A (control group), Group B (CCl4 experimental group), and Group C (CCl4 plus Raphanus sativus group). Blood samples were collected through cardiac puncture followed by hepatic histopathological analysis using light microscope. Data was analyzed using SPSS version 24, with ANOVA and Post hoc Tukey analysis used for comparison of different study variables.
Results: Statistically significant rise relative liver weight of group B rats compared with the group A and C rats (P <.05). Moreover, statistically significant (P <0.05) rise in serum markers of hepatic functions was observed after CCl4 administration in Group B. Treatment with Raphanus Sativus administration significantly reduced serum levels of LFTs (p<0.05). There was a significant decline in the plasma levels of oxidative markers in group B while marked histopathological changes like necrosis, sinusoidal dilatation and congestion observed among animals of group B.
Conclusion: Raphanus Sativus exerts an anti-oxidative, anti- and hepatoprotective effect against CCl4-induced hepatic tissue damage.
背景:莴苣是一种十字花科植物,具有清除自由基的特性,可以保护身体免受活性氧的有害影响。通过对白化Wistar大鼠肝功能及氧化应激指标的测定,探讨萝卜对ccl4诱导的肝毒性的保护作用。材料与方法:准实验研究于2019年9月至2020年3月在海德拉巴以色列大学研究生研究实验室进行。选择白化Wistar大鼠采用非随机目的抽样。将所有大鼠平均分为3组:A组(对照组)、B组(CCl4实验组)和C组(CCl4加莴苣组)。心脏穿刺采血,光镜下肝组织病理分析。数据分析采用SPSS version 24,不同研究变量的比较采用方差分析(ANOVA)和Post hoc Tukey分析。结果:B组大鼠肝脏相对重量较A、C组大鼠升高有统计学意义(P < 0.05)。b组大鼠给予CCl4后血清肝功能指标升高,差异有统计学意义(P <0.05)。给予莴苣治疗显著降低血清LFTs水平(P <0.05)。B组大鼠血浆中氧化标志物水平明显下降,B组大鼠出现坏死、窦扩张、充血等组织病理学改变。结论:莴苣对ccl4诱导的肝组织损伤具有抗氧化、抗肝保护作用。