Effect of Gonadotropins and Gonadal Steroids (17β-Estradiol and Testosterone) on Harderian Gland Porphyrin Content and Circulatory Hormones in Indian Palm Squirrel Funambulus pennanti

Abstract

Mostly studied in the hamster, the Harderian gland (HG, an extra-orbital gland) has been shown to be capable of melatonin (MEL) synthesis and to possess steroid receptor binding sites. Sexual dimorphism and ability to respond to steroid hormones have been reported for HG of some temperate zone mammals. However, to date there is no report on functional relation of HG with pineal, pituitary and gonad in any rodent. Hence, we tested the effect of gonadotropins [FSH (10μg) + LH (10μg)] and steroids [testosterone (100μg) and 17-β estradiol (50μg)] on HG function in relation to the activities of the pineal gland and gonad in both sexes of Indian palm squirrel Funambulus pennanti during the sexually quiescent phase. In general, squirrels treated with gonadotropins and gonadal steroids showed a significant increase in gonadal weight and decrease in pineal weight and plasma melatonin level in LH- and testosterone-treated groups only. There was no change in the weight of HG on FSH treatment. Porphyrin content of HG decreased after testosterone and estradiol treatment. Pineal gland weight invariably decreased after treatment with gonadotropins and testosterone. Plasma testosterone and estradiol levels increased but melatonin level decreased in all treated groups. Our data suggest that in this tropical rodent gonadotropins have an indirect effect on HG and that gonadal steroids have significant effect on HG through the influence on gonadal and pineal functions.