Cytological comparison of gill chloride cells and blood serum ion concentrations in kutum (Rutilus frisii kutum) spawners from brackish (Caspian Sea) and fresh water (Khoshkrood River) environments

Abstract

Abstract The size and number of chloride cells and serum ion concentrations in kutum, Rutillus frisii kutum Nordman, from brackish (Caspian Sea) and fresh water (Khoshkrood River) environments were studied to gain a better understanding of osmoregulation in this species. Twenty mature kutum specimens were collected from the Caspian Sea (Anzali coasts, 8.49 ppt salinity and 12.4°C temperature) and 20 specimens from Khoshkrood River (0.18 ppt salinity and 18°C temperature). Gill samples were analyzed histologically and concentrations of Na+, Cl- , K+, and Mg2+ ions were determined in the blood serum. Concentrations of Na+, Cl- , K+, and Mg2+ ions and osmotic pressure in mature kutum from brackish water were significantly higher than in specimens from fresh water. The average size and number of chloride cells in the fish from seawater were considerably larger than those from fresh water. The mean size of chloride cells was 6.89 ± 1.16 μm in brackish water samples and 5.1 ± 0.81 μm in river samples. The average number of chloride cells in brackish and river water samples were 16.92 and 6.57, respectively. The density and size of chloride cells increased with increases in salinity