COMPARISON AND VALIDATION OF A FAST METHOD FOR THE EXTRACTION AND QUANTIFICATION OF α-LINOLENIC, EICOSAPENTAENOIC, AND DOCOSAHEXAENOIC FATTY ACIDS

Abstract

BACKGROUND: Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds. OBJETIVE: In this paper the accuracy is verified on the fat extraction in fish, between two methods, using one of the most employees as it is the Bligh & Dyer since 1959, compared with SOXTEC, one more updated method with more modern equipment and validated quantification method for some fatty acids omega 3 by gas chromatography. METHODS: Fat extraction was performed by SOXTEC and Bligh & Dyer uses a Certified Reference Material (SRM 1946). After having assessed the accuracy of both systems, a quantification method of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids was validated through gas chromatography. RESULTS: It was found that the method proposed by Bligh & Dyer in 1959 is the most accurate because it showed better recovery percentages. Also, it was found that the omega 3 most important fatty acid quantification validation method complies with every parameter assessed throughout the validation. CONCLUSION: In conclusion Soxtec being a more modern method which uses the latest technology , the Bligh & Dyer is the most effective method for removing fat in fish and gas chromatography allows quantification of fatty acids with precision and accuracy as meets the parameters evaluated .