Callogenesis of Durio zibethinus using flower bud explant

Abstract

Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation due to their ability to induce callogenesis (69.2%). Combination of 45 gr/L sucrose and 0.8 ppm TDZ gave the best result in inducing callus growth. Most of the callus showed white colour and friable callus.Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation du...