STUDY ON THE DPPH FREE RADICAL-SCAVENGING ACTIVITY OF SALVIA NEMOROSA L. AT TWO GROWTH STAGES

Abstract

This study was designed to examine the DPPH free radical-scavenging activity, in different concentrations (0.025, 0.05, 0.07, 0.1, 0.2, 0.04 and 0.6) of methanolic extracts of Salvia nemorosa L. collected from the northwest of Iran (Zonouz and Ardabil regions) at two-stage of growth (vegetative stage leaves, flowering stage leaves, and flowers). The result showed that the mean of inhibition percentage in the Zonouz region increased in various concentrations and between flowers, vegetative stage leaves, and flowering stage leaves, compared with the plants of the Ardabil region. In each of the regions of Zonouz and Ardabil, the highest amount of DPPH inhibition was observed in the vegetative stage leaves in comparison with flowering stage leaves and flowers. In addition, in the effect of DPPH radical trapping in different concentrations of methanolic extracts of Salvia nemorosa L. was observed that from each of the collected region, methanolic extracts from sage plants were dose-dependent and acted very effective and useful and the best antioxidant activity was in the high concentration of extracts, So in Zonouz and Ardabil regions, the content of inhibition of DPPH increased significantly, by increasing the concentration of 0.025 mg/ml to 0.6 mg/ml and in Zonouz region the content of inhibition of DPPH similarly increased in 0.2, 0.4 and 0.6 mg/ml concentrations. In the Ardabil region, the most content of inhibition of DPPH was seen in 0.4 mg/ml and 0.6 mg/ml concentrations, but in this region, the content of inhibition of DPPH in 0.2 mg/ml concentration there was only in vegetative stage leaves and flowering stage leaves.