Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B

Abstract

This study aimed to investigate whether combined supplementation with vitamin C and vitamin E was able to modify the superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the ovarium of rats exposed to rhodamine B. Twenty-five female Wistar albino rats were divided into five groups (n = 5 each), including control (untreated group); rhodamine B group; rhodamine B group which received vitamin C (0.2 mg) + vitamin E (0.04 IU/g body weight); rhodamine B group which received vitamin C (0.4 mg) + vitamin E (0.04 IU/g body weight); and the rhodamine B group which received vitamin C (0.8 mg) + vitamin E (0.04 IU/g body weight). Analysis of MDA levels as a marker of lipid peroxidation was done spectrophotometrically. Analysis of SOD levels was done by enzyme-linked immunosorbent assay technically. Endometrial histology was analyzed in hematoxylin eosin staining. This increase in ovarian MDA was significantly (p < 0.05) attenuated by the two highest dose treatments of combined vitamin C and vitamin E. Rhodamine B significantly decreased SOD levels compared to the untreated group. This decrease in ovarian SOD level was significantly attenuated by the second and third doses of the combined vitamin C and vitamin E. The vascular number and gland density were significantly lower in the rhodamine B group compared to the untreated control group (p > 0.05). All doses also significantly prevented rhodamine B-induced decrease in the vascular number and gland density. In conclusion, the protective effect of combined vitamin C and vitamin E against ovarian and endometrial toxicity in rats receiving oral rhodamine B is due to inhibition of the lipid peroxidation, modulation of SOD levels, and the endometrium repairing effect.