Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using Drosophila S2 Cells

IF 0.3 4区农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY

Jarq-japan Agricultural Research Quarterly Pub Date : 2022-07-01 DOI:10.6090/jarq.56.245

Aki Sagisaka, R. Murakami, Kenji Watanabe, Hiromitsu Tanaka

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引用次数: 0

Abstract

Sap-sucking insects, such as aphids, leafhoppers, thrips, and whiteflies, are vectors for > 70% of plant viruses. Presently, the development of insecticide resistance is causing a problem in agriculture worldwide as control of these insect vectors and of virus transmission is increasingly difficult. Insects lack an adaptive immune response system but use RNA interference (RNAi) functions as antiviral defense systems. Nevertheless, some viruses that infect insects encode proteins termed as viral suppressors of RNA silencing (VSR) proteins that can act against the RNAi systems. VSR proteins enable viruses to keep and increase in number in the body of the insects. The present study aimed to construct a VSR detection system for use in the detection of VSRs of viruses transmitted by insect vectors. The activities of VSR proteins were measured using a dual-luciferase reporter assay in S2 Drosophila cells. The analyses showed that HC-pro protein from Zucchini yellow mosaic virus had the highest level of activity among the tested VSR proteins. This methodological approach enables the detection of VSR proteins and measurement of VSR activities of insect viruses as well as plant viruses transmitted by insect vectors.

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利用果蝇S2细胞检测昆虫传播病毒RNA干扰活性病毒抑制蛋白

蚜虫、叶蝉、蓟马和白蝇等吸液昆虫是70%以上植物病毒的传播媒介。目前，杀虫剂抗性的发展给世界范围内的农业造成了一个问题，因为控制这些昆虫媒介和病毒传播越来越困难。昆虫缺乏适应性免疫反应系统，但利用RNA干扰(RNAi)功能作为抗病毒防御系统。然而，一些感染昆虫的病毒编码的蛋白质被称为RNA沉默的病毒抑制因子(VSR)蛋白质，可以对RNAi系统起作用。VSR蛋白使病毒能够在昆虫体内保持和增加数量。本研究旨在建立昆虫媒介传播的VSR检测系统。采用双荧光素酶报告法测定S2果蝇细胞中VSR蛋白的活性。分析结果表明，西葫芦黄花叶病毒HC-pro蛋白在所有VSR蛋白中活性最高。该方法能够检测昆虫病毒以及通过昆虫媒介传播的植物病毒的VSR蛋白和VSR活性。

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来源期刊

Jarq-japan Agricultural Research Quarterly 农林科学-农业综合

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japan Agricultural Research Quarterly (JARQ) is a publication of the Japan International Research Center for Agricultural Sciences (JIRCAS), which provides readers overseas with the latest information on key achievements and developments in agricultural research in Japan, with the expectation that this information would contribute to the agricultural development of countries in tropical and subtropical regions.