Lipid circulation in spiders. Transport of phospholipids, free acids and triacylglycerols as the major lipid classes by a high-density lipoprotein fraction isolated from plasma of Polybetes pythagoricus

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-10-01 DOI:10.1016/0305-0491(94)90017-5

M. Cunningham, R. Pollero, A. González

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引用次数: 26

Abstract

A high-density lipoprotein (HDL) fraction was isolated from the hemolymphatic plasma of the spider Polybetes pythagoricus by density-gradient ultracentrifugation. Hydrated density (1.13 g/ml), electrophoretic mobility (SDS—PAGE) of apoproteins and lipid classes composition were determined. Lipids were identified by HP-TLC and auxiliary techniques; they were quantified by TLC-FID. The protein moiety is composed of two main apoproteins (250 and 76 kDa, respectively) and several polypeptides of low molecular weight. It resembles the apoliphorins of insects and some other arachnids. The lipid composition differs from most lipophorins. Phospholipids amount to more than 60% of total lipids, while diacylglycerols (2.4%) are supplanted by triacylglycerols (16.5%) as the main circulating energetic lipids.

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蜘蛛的脂质循环。从毕达哥拉斯甜菜血浆中分离的高密度脂蛋白部分转运磷脂、游离酸和三酰基甘油作为主要脂类

采用密度梯度超离心的方法，从毕达哥拉斯蜘蛛的血淋巴血浆中分离出高密度脂蛋白(HDL)。测定载脂蛋白的水合密度(1.13 g/ml)、电泳迁移率(SDS-PAGE)和脂类组成。脂质鉴定采用HP-TLC及辅助技术;用TLC-FID定量。该蛋白片段由两个主要载脂蛋白(分别为250和76 kDa)和几个低分子量多肽组成。它类似于昆虫和其他一些蛛形纲动物的载磷素。脂质组成不同于大多数脂磷脂。磷脂占总脂质的60%以上，二酰基甘油(2.4%)被三酰基甘油(16.5%)所取代，成为主要的循环能量脂类。

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

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