Nutritional Requirement of Pasteurella multocida

Abstract

From lactalbumin hydrolysate (enzymatically) peptide essential to the growth of Pasteurella multocida was isolated by gel filtration using Sephadex G-25 and by column chromatography with the use of Dowex 50W×2. Growth-eliciting effect of effective fraction separated with gel filtration was considerably below that of original material, although that of mixture of the all fractions obtained with gel filtration approximated to that of original material. The addition of various ineffective fractions to the effective one obtained with gel filtration led to different responses; one group of fractions stimulated bacterial growth, the other group of fractions was ineffective or inhibited the growth.