Automatic Focusing Using Halton Sampling

Abstract

A critical step in automated microscopy is the autofocusing algorithm. When focusing multiple fields of view, the variation of the focus plane in neighboring fields is low, and a subset of these can be used for focusing, estimating the rest by interpolation. The choice of the sampling points has an influence on the interpolation error. We introduce and test a new sampling algorithm based on the Halton set, which significantly improves the interpolation error.