Ultrasound-induced cell detachment and gene transfection in adherent cells

Xiaochun Lu, P. Zhong
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引用次数: 5

Abstract

The effect of 1-MHz cw ultrasound (0.36 MPa in pressure amplitude) on cell detachment and gene transfection in adherent HeLa cells was investigated. Cell detachment, cell injury, and gene transfection (up to 50%) in surviving populations were found to increase progressively with exposure duration (0–20 s). However, with respect to the total exposed cells, gene transfection efficiency in the adherent cells (3%) was significantly lower than its counterpart without detachment (15%). Cell detachment by ultrasound exposure was accompanied by significantly reduced proliferation rate with greatly increased apoptosis in the surviving detached cell population.
超声诱导细胞脱离及贴壁细胞的基因转染
研究了1 mhz连续波超声(压力幅值0.36 MPa)对贴壁HeLa细胞剥离和基因转染的影响。随着暴露时间的延长(0-20 s),存活群体的细胞脱离、细胞损伤和基因转染(高达50%)逐渐增加。然而,相对于暴露的总细胞,贴壁细胞的基因转染效率(3%)明显低于未分离的细胞(15%)。超声暴露下的细胞分离伴随着细胞增殖率的显著降低和细胞凋亡的显著增加。
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