Proteomics of the Oomycete Phytophthora parasitica Strain INRA 310

GM crops Pub Date : 2023-04-11 DOI:10.3390/crops3020011
Sihem Hannat, I. Hasni, P. Decloquement, S. Diene, S. Azza, B. La Scola, S. Aherfi
{"title":"Proteomics of the Oomycete Phytophthora parasitica Strain INRA 310","authors":"Sihem Hannat, I. Hasni, P. Decloquement, S. Diene, S. Azza, B. La Scola, S. Aherfi","doi":"10.3390/crops3020011","DOIUrl":null,"url":null,"abstract":"The phytopathogen Phytophthora parasitica, from the Oomycetes class, known to be the tobacco black shank agent, can induce devastating diseases in various crop, plant and forest ecosystems. The genus Phytophthora has been studied at the cellular level, suggesting that different developmental steps are induced by the expression of some specific genes. However, these studies have only been carried out on certain species, such as Phytophthora infestans and Phytophthora cactorum. As for Phytophthora parasitica, which can be considered as one of the top ten oomycete pathogens due to the economic impact and effect it has on food security, even less functional analyses and transcriptomics data are available. To date, little is known about the protein expression of Phytophthora parasitica, information that is essential for achieving a better understanding of this species. In this study, we aimed to gain insight into the proteomics of the mycelium of the Phytophthora parasitica strain INRA 310 by addressing the following questions: (i) how many predicted proteins can be detected on the mycelium of P. parasitica INRA 310, and (ii) what proteins can be detected? The proteomics experiments were performed on the mycelium of the strain Phytophthora parasitica INRA310, using the nanoliquid chromatography-MS/MS technique. A total of 219 proteins were identified, including ten unknown proteins and 209 proteins involved in lipid, carbohydrate, nucleotide, energy production and other metabolic pathways. This proteomics study is, to our knowledge, the first to be performed on the mycelium of Phytophthora parasitica INRA 310. It gives a brief first insight into its in vitro-expressed proteins. This work may be the first step before further, more comprehensive studies are undertaken with the aim of better understanding the biology of this species and its pathogenicity.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"13 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"GM crops","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/crops3020011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The phytopathogen Phytophthora parasitica, from the Oomycetes class, known to be the tobacco black shank agent, can induce devastating diseases in various crop, plant and forest ecosystems. The genus Phytophthora has been studied at the cellular level, suggesting that different developmental steps are induced by the expression of some specific genes. However, these studies have only been carried out on certain species, such as Phytophthora infestans and Phytophthora cactorum. As for Phytophthora parasitica, which can be considered as one of the top ten oomycete pathogens due to the economic impact and effect it has on food security, even less functional analyses and transcriptomics data are available. To date, little is known about the protein expression of Phytophthora parasitica, information that is essential for achieving a better understanding of this species. In this study, we aimed to gain insight into the proteomics of the mycelium of the Phytophthora parasitica strain INRA 310 by addressing the following questions: (i) how many predicted proteins can be detected on the mycelium of P. parasitica INRA 310, and (ii) what proteins can be detected? The proteomics experiments were performed on the mycelium of the strain Phytophthora parasitica INRA310, using the nanoliquid chromatography-MS/MS technique. A total of 219 proteins were identified, including ten unknown proteins and 209 proteins involved in lipid, carbohydrate, nucleotide, energy production and other metabolic pathways. This proteomics study is, to our knowledge, the first to be performed on the mycelium of Phytophthora parasitica INRA 310. It gives a brief first insight into its in vitro-expressed proteins. This work may be the first step before further, more comprehensive studies are undertaken with the aim of better understanding the biology of this species and its pathogenicity.
卵霉菌疫霉菌株INRA 310的蛋白质组学研究
来自卵菌纲的植物病原菌疫霉(Phytophthora parasitica)被称为烟草黑胫病菌,可在各种作物、植物和森林生态系统中引起毁灭性疾病。在细胞水平上对疫霉属进行了研究,表明不同的发育步骤是由一些特定基因的表达诱导的。然而,这些研究只在某些物种上进行,如疫霉菌和疫霉菌。由于对经济和粮食安全的影响,疫霉被认为是十大卵菌病原菌之一,但其功能分析和转录组学数据则更少。迄今为止,人们对疫霉的蛋白表达知之甚少,而这些信息对于更好地了解这个物种是必不可少的。在这项研究中,我们旨在通过解决以下问题来深入了解寄生疫霉菌株INRA 310菌丝体的蛋白质组学:(i)在寄生疫霉INRA 310菌丝体上可以检测到多少预测蛋白,(ii)可以检测到哪些蛋白?采用纳米液相色谱-质谱联用技术对疫霉菌INRA310菌丝体进行蛋白质组学研究。共鉴定出219个蛋白,其中未知蛋白10个,参与脂质、碳水化合物、核苷酸、能量产生等代谢途径的蛋白209个。据我们所知,这项蛋白质组学研究是第一次对疫霉INRA 310的菌丝体进行研究。它提供了一个简短的第一次洞察其体外表达的蛋白质。这项工作可能是进一步开展更全面的研究的第一步,目的是更好地了解该物种的生物学及其致病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信