Staphylococcal lipase: Intracellular enzyme production

Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... Pub Date : 1983-12-01 DOI:10.1016/S0174-3031(83)80065-1

Stefan Tyski , Waleria Hryniewicz, Janusz Jeljaszewicz

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Abstract

It is well known that staphylococci produce extracellular lipase which has been isolated, purified and characterized. Existence of intracellular lipase has not been yet established. In this investigation, cellular content liberated by lysostaphin digestion of cell wall revealed presence of lipolytic activity. This has been observed with living staphylococcal cells only. Presence of intracellular lipase was not observed in young bacterial cells, and the enzyme was appearing rapidly in the late phase of the culture. Highest level of enzyme was observed on the 3rd day of culture in described conditions. Accumulation of lipase inside cells followed by its liberation was not observed.

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葡萄球菌脂肪酶:胞内酶生产

众所周知，葡萄球菌产生胞外脂肪酶，已被分离、纯化和鉴定。细胞内脂肪酶的存在尚未确定。在这项研究中，溶葡萄球菌消化细胞壁释放的细胞内容物显示存在溶脂活性。这只在活的葡萄球菌细胞中观察到。在年轻的细菌细胞中未观察到细胞内脂肪酶的存在，该酶在培养后期迅速出现。在上述条件下，培养第3天酶水平最高。没有观察到脂肪酶在细胞内的积累和随后的释放。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...

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