AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAN FRAKSI HEKSANA DAUN SOYOGIK (Saurauia bracteosa DC) TERHADAP OKSIDASI ASAM LINOLEAT

IF 1 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

化学进展 Pub Date : 2021-11-30 DOI:10.35799/cp.14.2.2021.37559

M. R. Runtuwene, V. Kamu, Marsella Rotty

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引用次数: 0

Abstract

Soyogik (Saurauia bracteosa DC) merupakan tanaman obat yang berasal dari Minahasa Tenggara, Provinsi Sulawesi Utara. Penelitian ini bertujuan untuk menentukan potensi aktivitas antioksidan ekstrak dan fraksi pelarut dari daun soyogik. Ekstraksi dilakukan dengan maserasi menggunakan metanol dan fraksinasi dengan n-heksana dan etil asetat. Kandungan total fenolik ekstrak dan fraksi diukur menggunakan uji Folin-Ciocalteu sedangkan aktivitas antioksidan dievaluasi dengan penghambatan oksidasi asam linoleat dengan metode Ferric Thiocyanate (FTC) dan penghambatan malonaldehid (MDA) dengan metode Thiobarbituric Acid Reactive Substance (TBARS). Hasil penelitian menunjukkan bahwa kandungan fenolik yang dinyatakan dalam ekuivalen asam galat dari ekstrak dan fraksi berkisar antara 50,83-99,67 g/mL. Fraksi etil asetat memiliki kandungan fenolik yang lebih tinggi dibandingkan fraksi heksana dan ekstrak metanol. Hasil penelitian ini juga menunjukkan bahwa fraksi etil asetat, n-heksana, dan α-tokoferol memiliki penghambatan peroksida masing-masing sebesar 50,21, 44,26, dan 46,96% dan penghambatan malonaldehid masing-masing 91,03, 88,81, dan 89,48%. Kemampuan hambat peroksida dan malonaldehid menunjukkan bahwa fraksi etil asetat memiliki aktivitas antioksidan yang lebih tinggi dibandingkan fraksi n-heksana dan a-tokoferol sebagai kontrol positif.ABSTRACT Soyogik (Saurauia bracteosa DC) is a medicinal plant originating from Southeast Minahasa, North Sulawesi Province. The objective of study was to determine the potential antioxidant activity of the extract and the solvent fraction of soyogik leaves. Extraction was carried out by maceration using methanol and fractionation with n-hexane and ethyl acetate. The total phenolic contents of extract and fractions were measured using a Folin-Ciocalteu assay while the antioxidant activities was evaluated by the inhibition of linoleic acid oxidation with the Ferric Thiocyanate (FTC) and measuring the malonaldehyde (MDA) inhibition with the Thiobarbituric Acid Reactive Substance (TBARS) methods. The results showed that phenolic content expressed in gallat acid equivalent of extract and fractions ranged from 50.83-99.67 µg/mL. The ethyl acetate fraction has higher phenolic content than hexane fraction and methanol extract. The results showed also that the ethyl acetate, n-hexane fractions and a-tocopherol had peroxide inhibition were 50.21, 44.26, and 46.96%, respectively and the inhibition of malonaldehyde were 91.03, 88.81, and 89.48% respectively. The inhibitory ability of peroxides and malonaldehyde showed that the ethyl acetate fraction had higher antioxidant activity than the n-hexane and a-tocopherol fractions as positive controls.

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乙基乙酸乙酯和丙酮的抗氧化剂活性(Saurauia bracteosa直属)对油酸氧化的抗氧化物成分

Soyogik (Saurauia bracteosa)是一种药用植物，它来自北苏拉威西省米纳哈萨东南部。本研究旨在确定抗氧化物提取物的潜在活性和溶剂的溶质成分。提取方法是用甲醇、炼金术、n-heksana和醋酸乙酯进行。萃取物和微量成分的总表型含量和微量成分是用氟硝酸(FTC)的苯甲酸氧化抑制(FTC)和马宏德希德(MDA)的脱氧核糖核酸反应性物质(TBARS)进行评估的。研究结果表明，提取物中所述的酚含量和微量成分约为50.83 - 99.67 g/mL。醋酸乙酚含量高于heksana和甲醇提取物。这些研究结果也表明:醋酸纤维素乙醇成分、n-heksana和α-tokoferol有抑制过氧化氢分别高达50.21 44.26,46,96%和抑制malonaldehid 91.03 88.81,,每根89,48%。过氧化氢和马宏德希德的抑制能力表明，醋酸乙滴的抗氧化活性高于n-heksana和a-tokoferol为积极控制。ABSTRACT Soyogik (Saurauia bracteosa)是一种来自北苏拉威西省东南部米纳哈萨的medicinal plant。研究的目的是确定潜在的抗氧化剂、extract和溶剂能叶子的成分。macertion使用甲烷和对n-hexane和ethyl acetate进行发酵。总表象和外部关系的表象使用了四小时的证据，而反氧活动的证据是由二氧乙酸(FTC)和用硫乙酰乙酸(MDA)装饰马宏生(TBARS)的树脂反应物质(TBARS)抑制的。《gallat results那里那个酚内容expressed酸哪里extract fractions著作百科全书》ranged从50 83-99。67µg / mL。乙基的吸引力比hexane的吸引力和甲烷排泄物更强。结果还显示，乙基溶液、n-hexane fractions和a-tocopherol的过氧化氢抑制为50.21,44.26和46.96%，尊重和马隆海德的抑制为91.03 88.81和88.48%的尊重。变质和变质海德的抑制作用表明，乙酰乙胺和抗氧化剂的强度比正控制的n-hexane和a-tocopherol帧低得多。

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来源期刊

化学进展化学-化学综合

CiteScore

2.10

自引率

15.40%

发文量

4325

审稿时长

2.5 months

期刊介绍： Progress in Chemistry is a peer-reviewed monthly journal. It is sponsored by the Chinese Academy of Sciences(CAS) and the National Natural Science Foundation of China(NSFC). It provides a forum to publish review papers of specialized topics covering the full spectrum of chemistry in Chinese or English, with emphasis on those topics of emerging research area. The reviews provide comprehensive information, including recent advances, development trends, as well as critical assessments about the subject. The readers interested are : researchers and students in chemistry and related areas, and policy decision-makers. Most members of the editorial board are famous scientists.