Effect of temperature on hydrolysis of triolein by lipase

Abstract

Effects of temperature on both the progress curves and reaction rates of the separate steps of triolein hydrolysis: decomposition of tri-, di- and monoolein as well as production of oleic acid by lipase (Candida cylindracea) in AOT/isooctane reversed micellar systems were investigated. The activation energy determined from the Arrhenius plot of fatty acid production was 22.3 kJ/mol.