Isolation, fractionation and HPLC analysis of neutral phenolic compounds in rapeseeds

Abstract

HPLC has been applied for the analysis of neutral phenolic compounds in rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic compounds by solid phase extraction with a C 18 -cartridge and HPLC separation of the extracts so obtained are described. Additionally, a rapeseed extract was fractionated by a simple chromatographic method using Sephadex LH-20. Six main compounds from the extract were isolated. A further purification was performed by preparative HPLC and a C 18 -cartridge are used to isolate the neutral phenolic compounds. An attempt to identify the compounds was made from the UV-spectra corresponding to each isolated peak of the chromatogram.