Optimal fabrication condition of bacteriorhodopsin films by electrophoretic sedimentation technique

Abstract

The photocurrent of bacteriorhodopsin (bR) films fabricated by electrophoretic sedimentation (EPS) technique was investigated. The fabrication conditions by EPS, the intensity of electric field and exposure time, affected the photoelectric response and topology of bR films. Based on the photoelectric response and surface topology measured by an atomic force microscope (AFM), the optimal electric field for the fabrication of oriented bR film was 20 V/cm. The exposure time of 45 s did not affect the photocurrent of bR film but longer exposures damaged the bR films. The action spectrum of bR films fabricated by EPS technique was well consistent to the absorption spectrum of bR solution.