Performance of Various Organic Solvents as Reaction Media in Plant Oil Lipolysis with Plant Lipase

Reaktor Pub Date : 2018-08-24 DOI:10.14710/REAKTOR.18.2.71-75

A. N. Istyami, T. Soerawidjaja, T. Prakoso, Tri Ari Penia Kresnowati

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Abstract

Fatty acids are intermediate substances in synthesis of oleochemical products. Enzymatic technology of fatty acids production (also known as lipolysis) is now developing as potential substitution for the conventional production of fatty acid, i.e. thermal hydrolysis of triglyceride. It offers more economical process condition, low energy consumption, and minimal product degradation compared to the conventional process. This research aims to evaluate performance of various organic solvents as reaction media in lipolysis with plant latex lipase. Organic solvents observed were chloroform, n-hexane, diethyl ether, benzene, acetone, ethanol, methanol, n-heptane, and isooctane. Analysis of each organic solvent effect on lipolysis was described based on solvents properties. Conversion of lipolysis with organic solvents is 0,10-1,25 times fold compared to conversion of non-solvent lipolysis. We suggest that dielectric constant and viscosity are the two main organic solvent properties affecting lipase performance in lipolysis. Overall, n-hexane, n-heptane, and isooctane are recommended to be used as reaction media in lipolysis with plant lipase because their effects to degree of lipolysis are positive. Keywords: lipolysis; lipase; organic solvent; frangipani

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不同有机溶剂作为植物脂肪酶裂解植物油的反应介质的性能

脂肪酸是油脂化学产物合成中的中间物质。脂肪酸生产的酶技术(也称为脂解)目前正在发展，作为传统脂肪酸生产的潜在替代品，即热水解甘油三酯。与传统工艺相比，它提供了更经济的工艺条件，能耗低，产品降解最小。本研究旨在评价不同有机溶剂作为反应介质在植物乳胶乳脂肪酶水解油脂中的性能。观察到的有机溶剂有氯仿、正己烷、乙醚、苯、丙酮、乙醇、甲醇、正庚烷和异辛烷。从溶剂性质出发，分析了各种有机溶剂对脂肪分解的影响。与非溶剂脂肪分解的转化率相比，有机溶剂脂肪分解的转化率是0.10 - 1.25倍。我们认为介电常数和粘度是影响脂肪酶在脂肪分解过程中性能的两个主要有机溶剂性质。综上所述，正己烷、正庚烷和异辛烷对植物脂肪酶的脂解程度有积极的影响，因此推荐使用它们作为反应介质。关键词:脂类分解;脂肪酶;有机溶剂;鸡蛋花

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Reaktor

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2 weeks