Characteristics of fresh and frozen semen of Red Chittagong Cattle

Tanzil Mahmud Arefin, A. Bhuiyan, M.A.M. Yahia Khandoker, M. Habib, S. Das
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引用次数: 2

Abstract

Semen production from breeding bulls used in Artificial Insemination (AI) program is of great importance in cattle breeding. Good quality semen has positive effect on the fertility of cows and heifers. The aim of this study was to evaluate the quality and assessed as the freezing ability of Red Chittagong Cattle (RCC) bull with fresh-diluted semen and frozen semen. The parameters of semen characteristics included were volume per ejaculate (ml), percentage of mass motility, normal and abnormal sperm, and sperm concentration (million/ml). The highest mass motility was found in bull ID No. 4 and the lowest in bull ID No. 1 but the highest sperm concentration was found in bull ID No. 3 with lowest in bull ID No. 2 in fresh semen. Highly significant (p<0.001) positive correlation was found between mass motility and sperm concentration in bulls of ID No. 3 and 4, a negative significant (p<0.05) correlation was found between mass motility and normal sperm percentage. Two freezing techniques were followed in frozen semen production: Cork sheet freezing and Cryobath freezing with two freezing times 15 and 30 minutes were also applied. Variations in the motility of frozen semen by Cork sheet freezing with two different times were found significant (p<0.05) between bulls. Furthermore, highly significant (p<0.01) difference of motility by Cryobath freezing was found between the bulls. The highest motility after both 15 and 30 minutes Cork sheet freezing and Cryobath freezing was found in bull ID No. 4 and lowest in bull ID No. 3.  In the present study, variability in the potentiality of bulls (semen volume and sperm concentration) was observed and found lower than crossbred bulls due to small size of Red Chittagong, an indigenous zebu cattle of Bangladesh. Bangladesh Journal of Animal Science. 51(1):25-30  
吉大港红牛鲜、冻精液的特性
用于人工授精(AI)计划的种牛精液生产在牛的育种中具有重要意义。优质精液对牛、小母牛的生育能力有积极的影响。本研究对吉大港红牛(RCC)公牛的新鲜稀释精液和冷冻精液进行了质量评价和冷冻性能评价。精液特征参数包括每次射精量(ml)、质量能动性百分比、正常和异常精子、精子浓度(百万/ml)。公牛4号的质量活动力最高,公牛1号最低,而公牛3号的精子浓度最高,公牛2号最低。3号和4号公牛的质量活动力与精子浓度呈极显著正相关(p<0.001),与正常精子百分比呈显著负相关(p<0.05)。冷冻精液生产采用两种冷冻技术:软木片冷冻和冷冻浴冷冻,冷冻时间分别为15分钟和30分钟。不同时间软木薄片冷冻后的精液活力在公牛间差异显著(p<0.05)。此外,在低温冷冻条件下,两种公牛的运动能力差异极显著(p<0.01)。15和30分钟软木薄片冷冻和低温冷冻后,4号公牛的活力最高,3号公牛的活力最低。在本研究中,观察到公牛的潜力(精液量和精子浓度)的变异性,并发现由于红色吉大港(孟加拉国土著zebu牛)体型较小,公牛的潜力低于杂交公牛。动物学报。51(1):25-30
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