Association between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery

Medical laboratory sciences Pub Date : 2020-11-29 DOI:10.22037/AMLS.V6.31298

M. Esmailidehaj, S. Mirhosseini, Zakieh Sadat Sheikhalishahi

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Abstract

Background and Aim: Left ventricular dysfunction is a frequent complication after coronary artery bypass graft (CABG) and is often treated with inotropic drugs to achieve suitable hemodynamic status. Bone marrow-derived mesenchymal stem cells (BMSCs) have potential effects on cardiac function. In this study, we aimed to identify the predictor role of MSCs in taking inotropic drugs in patients with severe left ventricular dysfunction undergoing CABG. Methods: The study included 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For investigating the possible association of BMSCs proliferation rate with taking inotropic drugs, the bone marrow samples aspired from patients' sternum during surgery. MSCs were isolated and counted after 4, 7, and 14 days using trypan-blue color, and then doubling times were calculated. Results: After cardiac surgery, the number of female patients who take inotropic drugs was significantly higher than men. Our data showed that the BMSCs doubling time in female patients who received inotropic drugs was less than that of male patients who received inotropic drugs (p < 0.05). Conclusion: Based on this investigation, we concluded that there was a clear relevance between the MSCs' doubling time and the inotropic drug requirements in patients who received inotropic drugs. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com Please cite this article as Esmailidehaj M, Mirhosseini S.J, Sheikhalishahi Z.S. Association Between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery. Arch Med Lab Sci. 2020;6:1-6 (e14). https://doi.org/10.22037/amls.v6.31298

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非体外循环手术后严重左心功能不全患者骨髓间充质干细胞特性与服用肌力药物的关系

背景与目的:左心室功能不全是冠状动脉搭桥术(CABG)术后常见的并发症，常应用肌力药物治疗以达到合适的血流动力学状态。骨髓间充质干细胞(BMSCs)对心脏功能有潜在的影响。在这项研究中，我们旨在确定MSCs在严重左心室功能障碍患者行冠脉搭桥时服用肌力药物的预测作用。方法:本研究包括30例在Afshar医院和Seyed Al-Shohada医院接受体外循环冠脉搭桥的患者。为了研究骨髓间充质干细胞增殖率与服用肌力药物的可能关联，在手术期间从患者胸骨抽取骨髓样本。分别于4、7、14天后分离MSCs，用台盼蓝染色计数，计算翻倍次数。结果:心脏手术后，女性患者服用肌力药物的人数明显高于男性。我们的数据显示，接受肌力药物治疗的女性患者骨髓间充质干细胞倍增时间少于接受肌力药物治疗的男性患者(p < 0.05)。结论:基于这项研究，我们得出结论，在接受肌力药物治疗的患者中，MSCs的倍增时间与肌力药物需求之间存在明显的相关性。*通讯作者:Zakieh Sadat Sheikhalishahi;Esmailidehaj M, Mirhosseini S.J, Sheikhalishahi z.s。骨髓间充质干细胞特征与非体外循环手术后严重左心室功能障碍患者服用肌力药物的关系。中华医学杂志，2020;6:1-6 (e14)。https://doi.org/10.22037/amls.v6.31298

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Medical laboratory sciences

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