In Vitro Evaluation of Cytotoxicity Effect of Ihau Fruit Extract (Dimocarpus longan var. Malesianus Leenh.) on MCF-7 Breast Cancer Cell Line

I. Rahmawati, Ghina Putri Dyanti, R. Widyanto, Annisa Rizky Maulidiana, Wyna Nabila, R. Purwestri
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Abstract

Cancer prevalence is steadily increasing each year and becomes the second leading cause of death worldwide. In Indonesia, breast cancer had a prevalence of 16.7% in 2018. Free radicals contribute to the occurrence of breast cancer, while antioxidants play a vital role in protecting cells and repairing the damage caused by free radicals. Ihau, an endemic fruit in Kalimantan Island, contains phytochemical compounds with potential antioxidant and anticancer properties. Utilizing local food as natural antioxidants could serve as an alternative for breast cancer prevention and treatment. This study aims to assess the anticancer potential of Ihau fruit extract on the MCF-7 breast cancer cell line. A post-test-only control group design method using the MTT assay was used, with two treatment groups: water and 96% ethanol extract, and four different concentrations (125, 250, 500, and 1000 ppm), and each was replicated three times. Statistical analysis using the ANOVA test found no significant difference among all concentrations. The IC50 values of the cytotoxic activity of water and ethanol extracts were 1,197.7 ppm and 1,148 ppm, respectively. It can be concluded that both water and ethanol extract of Ihau fruit exhibited very weak cytotoxic activity.
龙眼果提取物对MCF-7乳腺癌细胞毒性的体外研究
癌症发病率每年都在稳步上升,并成为全球第二大死亡原因。2018年,印度尼西亚的乳腺癌患病率为16.7%。自由基有助于乳腺癌的发生,而抗氧化剂在保护细胞和修复自由基造成的损伤方面起着至关重要的作用。Ihau是加里曼丹岛的一种特有水果,含有具有潜在抗氧化和抗癌特性的植物化学化合物。利用当地食物作为天然抗氧化剂可以作为乳腺癌预防和治疗的替代选择。本研究旨在探讨Ihau果提取物对MCF-7乳腺癌细胞系的抗癌作用。采用MTT法的后验对照组设计方法,采用水和96%乙醇提取物两个处理组,四种不同浓度(125、250、500和1000 ppm),每个重复3次。采用方差分析(ANOVA)进行统计分析,发现各浓度间无显著差异。水提取物和乙醇提取物的细胞毒活性IC50值分别为1197.7 ppm和1148 ppm。结果表明,水提取物和乙醇提取物均表现出极弱的细胞毒活性。
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