Production of Thermostable a-amylase by Bacillus cereus MK in solid state fermentation: Partial purification and characterization of the enzyme

S. Mrudula, R. Kokila
{"title":"Production of Thermostable a-amylase by Bacillus cereus MK in solid state fermentation: Partial purification and characterization of the enzyme","authors":"S. Mrudula, R. Kokila","doi":"10.5580/1542","DOIUrl":null,"url":null,"abstract":"Thermostable a-amylase production under solid state fermentation was investigated using isolated thermophilic Bacillus cereus MK. Optimization of process parameters followed by leaching parameters for enhanced enzyme yields were carried out. The optimum temperature, pH, incubation period, inoculation size and substrate to moisture ratio were found to be 55 C, 7.0, 24 h, 15 % (v/w) and 12.5 (w/v), respectively. Among different carbon, nitrogen and trace elements supplemented, glucose, peptone and calcium chloride, respectively enhanced enzyme production. The optimum leaching parameters such as solvents, solvent volume, physical state, solvent temperature and solvent pH for effective extraction of amylase from the fermented bran were found to be sodium acetate buffer (0.1M, pH 5.6), 1:2.5 (w/v), agitation, 50 C and 7.0, respectively. An overall 14 fold increase in enzyme production was attained by optimization of process conditions and leaching parameters in SSF. The crude amylase showed maximum activity at pH 7.0 and 90 C. The enzyme was stable at 90 C for 1h. However in the presence of 4% (w/v) starch, the stability of enzyme was increased to100 C up to 2h.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1542","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15

Abstract

Thermostable a-amylase production under solid state fermentation was investigated using isolated thermophilic Bacillus cereus MK. Optimization of process parameters followed by leaching parameters for enhanced enzyme yields were carried out. The optimum temperature, pH, incubation period, inoculation size and substrate to moisture ratio were found to be 55 C, 7.0, 24 h, 15 % (v/w) and 12.5 (w/v), respectively. Among different carbon, nitrogen and trace elements supplemented, glucose, peptone and calcium chloride, respectively enhanced enzyme production. The optimum leaching parameters such as solvents, solvent volume, physical state, solvent temperature and solvent pH for effective extraction of amylase from the fermented bran were found to be sodium acetate buffer (0.1M, pH 5.6), 1:2.5 (w/v), agitation, 50 C and 7.0, respectively. An overall 14 fold increase in enzyme production was attained by optimization of process conditions and leaching parameters in SSF. The crude amylase showed maximum activity at pH 7.0 and 90 C. The enzyme was stable at 90 C for 1h. However in the presence of 4% (w/v) starch, the stability of enzyme was increased to100 C up to 2h.
蜡样芽孢杆菌MK固态发酵生产耐热a-淀粉酶:酶的部分纯化和特性
利用分离的嗜热蜡样芽孢杆菌(Bacillus cereus)进行固态发酵产耐热a-淀粉酶的研究,并对工艺参数和浸出参数进行了优化,以提高酶的产量。结果表明,最适温度为55℃,最适pH为7.0,最适孵育时间为24 h,最适接种量为15% (v/w),最适基质水分比为12.5 (w/v)。在添加不同碳、氮和微量元素的情况下,葡萄糖、蛋白胨和氯化钙分别促进了酶的生成。从发酵麸皮中有效提取淀粉酶的最佳浸出参数为溶剂、溶剂体积、物理状态、溶剂温度和溶剂pH,分别为醋酸钠缓冲液(0.1M, pH 5.6)、1:25 .5 (w/v)、搅拌、50℃和7.0℃。通过优化SSF的工艺条件和浸出参数,酶产量提高了14倍。粗淀粉酶在pH 7.0和90℃时活性最高,酶在90℃下稳定1h。而在4% (w/v)淀粉的存在下,酶的稳定性提高到100℃至2h。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信