Activity of Aristolochia bracteolata against Moraxella catarrhalis

International Journal of Bacteriology Pub Date : 2014-09-28 DOI:10.1155/2014/481686

Malik Suliman Mohamed, Mona Timan Idriss, A. Khedr, Haidar Abd AlGadir, S. Takeshita, M. Shah, Y. Ichinose, T. Maki

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引用次数: 15

Abstract

A bioassay-guided fractionation of methanol extract of Aristolochia bracteolata whole plant was carried out in order to evaluate its antimicrobial activity and to identify the active compounds in this extract. Antibacterial and antifungal activities of methanol extract against gram-positive, gram-negative, and fungal strains were investigated by the agar disk diffusion method. Among the strains tested, Moraxella catarrhalis and sea urchin-derived Bacillus sp. showed the highest sensitivity towards the methanol extract and hence they are used as test organisms for the bioassay-guided fractionation. From this extract, aristolochic acid 1 (AA-1) has been isolated and has showed the greatest antibacterial activity against both standard strain and clinical isolates of Moraxella catarrhalis with equal minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 25 and 50 μg/mL. Modification of the AA-1 to AA-1 methyl ester completely abolished the antibacterial activity of the compound and the piperonylic acid moiety of AA-1 which suggested that the coexistence of phenanthrene ring and free carboxylic acid is essential for AA-1 antibacterial activity.

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小马兜铃对卡他莫拉菌的抑制作用

采用生物测定法对小肩叶马兜铃甲醇提取物进行分离，以评价其抑菌活性，并鉴定其活性成分。采用琼脂盘扩散法研究甲醇提取物对革兰氏阳性、革兰氏阴性和真菌的抑菌和抑菌活性。在测试的菌株中，卡他莫拉菌和海胆来源的芽孢杆菌对甲醇提取物的敏感性最高，因此它们被用作生物测定引导分离的试验生物。从该提取物中分离得到马兜铃酸1 (AA-1)，对卡他莫拉菌标准菌株和临床分离株均表现出最强的抑菌活性，最低抑菌浓度(MIC)为25 μg/mL，最低杀菌浓度(MBC)为50 μg/mL。将AA-1修饰为AA-1甲酯后，化合物的抑菌活性和AA-1的胡椒酰酸部分完全消失，这表明菲环和游离羧酸的共存是AA-1抑菌活性的必要条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Bacteriology

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