Patterning technique of adhesive cells using ultrasound flexural vibration

Abstract

This paper investigated a cell patterning technique using ultrasound vibration. The ultrasound cell culture dish consisted of a culture dish with a glass bottom and a glass disc with an ultrasound transducer that generated a resonance flexural vibration mode on the bottom of the dish. HeLa cells were used as adhesives cells, and the growth of cells on the dish was observed under ultrasound excitation for 24 hours. The vibration amplitude was largest at the center of the dish, and the axisymmetric resonance flexural vibration mode with three concentric nodal circles and no nodal line was generated on the bottom of the dish. Large vibrations inhibited the cell growth. The distributions of the sound pressure amplitude and the acoustic radiation force in the culture medium were calculated with finite element analysis and it was found that the cell growth depended strongly on both the acoustic field in the culture medium and the vibration distribution of the dish. The ultrasound vibrations did not affect the viability of the cells, and the cell growth could be controlled by the flexural vibration of the cultured dish.This paper investigated a cell patterning technique using ultrasound vibration. The ultrasound cell culture dish consisted of a culture dish with a glass bottom and a glass disc with an ultrasound transducer that generated a resonance flexural vibration mode on the bottom of the dish. HeLa cells were used as adhesives cells, and the growth of cells on the dish was observed under ultrasound excitation for 24 hours. The vibration amplitude was largest at the center of the dish, and the axisymmetric resonance flexural vibration mode with three concentric nodal circles and no nodal line was generated on the bottom of the dish. Large vibrations inhibited the cell growth. The distributions of the sound pressure amplitude and the acoustic radiation force in the culture medium were calculated with finite element analysis and it was found that the cell growth depended strongly on both the acoustic field in the culture medium and the vibration distribution of the dish. The ultrasound vibrations did not affect the v...