Metabolic Characteristics of Invasive Plant Ipomoea cairica in South China by de novo Transcriptomics

热带亚热带植物学报 Pub Date : 2016-01-01 DOI:10.11926/J.ISSN.1005-3395.2016.02.002

Yan-Fei Geng, Ling-ling Chen, Huan Lu, C. Ning, L. Björn, Shaoshan Li

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引用次数: 5

Abstract

To explore the molecular mechanisms of Ipomoea cairica invasiveness insouth China, the de novo transcriptomes from I. cairica and two related species, I. digitata and I. nil,were sequenced and assembled.There were5 6551 all-unigenes obtained by assembling the reads, among them 56522 all-unigenes were annotated, including 7815, 15615, and 180201 all-unigenes in GO, COG and KEGG databases, respectively. Moreover, the activities of NR and GS in I.cairica, keyenzyme in metabolic pathway for nitrogen, were greater than those in related species. In addition, the transcriptome data showed that the genes of key enzymes in secondary metabolism, such aspal, 4cl, cad, chs, and chi, had synergic differential expressionin I. cairica, I. digitata and I. nil. The production synthesis from metabolic pathway could play a key role in stress-resistant, growth and allelopathy of I. cairica. The RT-qPCR verification results of key genes were similar to those from transcriptome.Therefore, the result of the present research might explain partly the successful invasiveness of I. cairicain South China at the level of molecular biology. (Less)

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利用从头转录组学研究华南入侵植物油豆的代谢特征

为探讨中国南方褐刺瓢虫(Ipomoea cairica)入侵的分子机制，对褐刺瓢虫(iomoea cairica)及其近缘种I. digitata和I. nil的从头转录组进行了测序和组装。组装reads共获得56551条all-unigenes，其中56522条all-unigenes被注释，其中GO、COG和KEGG数据库中的all-unigenes分别为7815条、15615条和180201条。此外，黄颡鱼氮代谢途径关键酶NR和GS活性高于近缘种。此外，转录组数据显示，次生代谢关键酶aspal、4cl、cad、chs、chi等基因在石竹、石竹和石竹中有协同差异表达。代谢途径的产物合成在核桃的抗逆性、生长和化感作用中起关键作用。关键基因的RT-qPCR验证结果与转录组结果相似。因此，本研究的结果可能在分子生物学水平上部分解释凯里凯里虫成功入侵华南的原因。(少)

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来源期刊

热带亚热带植物学报

自引率

0.00%

发文量

2589

期刊介绍： The Journal of Tropical Subtropical Botany, founded in 1991 and co-sponsored by the South China Botanical Garden of the Chinese Academy of Sciences and the Guangdong Provincial Botanical Society, is a bimonthly national academic journal in Chinese with domestic and international circulation. It gives priority to reporting new discoveries, theories, methods and technologies in the fields of botany, ecology, environmental sciences and their interdisciplinary fields in the tropical subtropical regions, and welcomes scientific research papers on plants from different climatic zones. It also welcomes research papers on plants from different climatic zones, focusing on global climate change and ecosystem services, systems and evolutionary biology, environmental degradation and ecological restoration, biodiversity conservation and sustainable use, agricultural and food quality and safety, and phytochemical resources, plant germplasm innovation and gene discovery and utilisation, as well as the development and utilisation of energy plants. There are columns of research reports, express reports, reviews, commentaries, book reviews and conference news. The journal is a core journal of natural sciences in China and publishes some English manuscripts. It has become an academic journal with significant influence in the fields of botany, ecology and its intersection in the tropical subtropical region of China, and has a certain degree of visibility in the international arena. It has been successively included in BIOSIS, CAS, CAB, Chinese Science Citation Database and Chinese Biological Abstracts.