Relationship of alleles to stutter ratios in Y-STR typing using the YfilerTM Plus PCR Amplification Kit

Abstract

 STAGE DOI: 10.3408 / jafst.764 ) Discrimination between allele peaks and stutter peaks is important in STR typing for forensic purposes. In minus stutter, it is known that there are correlations between alleles and stutter ratios, but there are few reports on other types of stutter such as plus stutter. In this study, we examined the relationship of alleles to stutter ratios in Y-STR typing using the Yˆler TM Plus PCR Ampliˆcation Kit. DNA was extracted from blood samples collected from Japanese males and multiplex PCR ampliˆcation was performed with 1 ng DNA template. In minus stutter, high correlation coe‹cients ( r ＞ 0.7 ) between alleles and stutter ratios were observed in 23 of 25 markers. Meanwhile, in other types of stutter, only the forward stutter in DYS392 showed such a high correlation coe‹cient. These results can be used for interpretation