Determination of HIF1Ars11549465 Polymorphism in Elite Skiers

Ömer Kaynar, Fatih Bilici, K. Ulucan, C. Sercan
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引用次数: 1

Abstract

ABSTRACT Studies in sport genetics have increased nowadays and the identification of genotype and allelic distributions of candidate genes in different athletes has provided important information to sports scientists. One of the candidate genes studied in sports genetics is the hypoxia-inducible factor 1-alpha (HIF1A) gene, which is associated with the endurance phenotype. In our study, we aimed to determine the genotypic and allelic distribution of HIF1A rs11549465 polymorphism in Turkish Elite Ski Runners. 34 (23 male, 11 female) athletes were included in the study, and 1 cc of blood samples taken for routine analysis after the approval of the ethics committee was used for DNA isolation. The commercial kit was used for DNA isolation and genotyping was performed by Real-time PCR method. The number and percentage of CC, CT and TT genotypes of the HIF1A rs11549465 polymorphism were 24 (72.72%), 8 (24.24%) and 1 (3.03), respectively. In male skiers CC, CT and TT genotype numbers and percentages were determined as 17 (55.51%), 4 (12.12%) and 1 (3.03%) respectively. In female, CC and TT genotypes were determined as 7 (21.21%) and 4 (12.12%). We detected no TT genotype in female athletes. When we look at allel numbers, C allele was determined as 56 (84.84%) and T allele was determined as 10 (15.15%). This is the first study on HIF1A rs11549465 polymorphism on Turkish elite ski runners. The higher prevalence of CC genotype and C allele in the athlete group is similar to the previous studies on different groups of athletes. We believe that this study will be an important reference for further studies.
优秀滑雪运动员HIF1Ars11549465多态性的测定
运动遗传学的研究日益增多,确定不同运动员候选基因的基因型和等位基因分布为运动科学家提供了重要信息。运动遗传学研究的候选基因之一是与耐力表型相关的缺氧诱导因子1- α (HIF1A)基因。本研究旨在确定土耳其优秀滑雪运动员中HIF1A rs11549465多态性的基因型和等位基因分布,研究对象为34名运动员(男23名,女11名),经伦理委员会批准,取血样1 cc进行常规分析,进行DNA分离。采用商业试剂盒进行DNA分离,Real-time PCR法进行基因分型。HIF1A rs11549465多态性CC、CT和TT基因型分别为24例(72.72%)、8例(24.24%)和1例(3.03)。男性滑雪者CC、CT和TT基因型分别为17例(55.51%)、4例(12.12%)和1例(3.03%)。女性CC和TT基因型分别为7(21.21%)和4(12.12%)。我们在女运动员中未检测到TT基因型。当我们看列数时,C等位基因确定为56 (84.84%),T等位基因确定为10(15.15%)。这是对土耳其优秀滑雪运动员HIF1A rs11549465多态性的首次研究。CC基因型和C等位基因在运动员群体中较高的患病率与以往对不同运动员群体的研究相似。我们相信本研究将为进一步的研究提供重要参考。
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