Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-04-20 DOI:10.1080/15592324.2022.2065432

Yong Li, Lingling Wu, Meiyan Ren, Jianshu Zhu, Jiming Xu, Han Hu, Xiaokang Quan, Chongping Huang, C. Mao

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引用次数: 1

Abstract

ABSTRACT The OsPIN1 paralogous genes (OsPIN1a-1d) are important for root and panicle development in rice (Oryza sativa L.). However, the specific role of OsPIN1 paralogous genes is still not clear. To understand the specific roles of PIN1 paralogs in rice, we generated pin1 triple and quadruple mutants by crossing the pin1a pin1b and pin1c pin1d double mutants which we previously created. Compared with the 7-day-old wild type, the pin1a pin1c pin1d and pin1b pin1c pin1d triple mutants showed no obvious phenotype variation except that the pin1a pin1c pin1d triple mutant had shorter primary root and shoot. The pin1a pin1b pin1c and pin1a pin1b pin1d triple mutants exhibited a series of developmental abnormalities, including shorter primary roots, longer root hairs, fewer crown roots and lateral roots, shorter and curved shoots. Furthermore, the pin1a pin1b pin1c pin1d quadruple mutant displayed more severe phenotypic defects which was lethal. In addition, the expression levels of some hormone signal transduction and crown root development related genes, such as OsIAAs, OsARFs, OsRRs, and OsCRLs, were significantly altered in the stem base of all examined pin1 multiple mutants. Taken together, our results demonstrated that the four OsPIN1 paralogous genes function redundantly in regulating rice growth and development.

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OsPIN1旁系基因在水稻生长发育调控中的功能冗余

摘要OsPIN1副同源基因(OsPIN1a-1d)在水稻根和穗发育中起重要作用。然而，OsPIN1旁系基因的具体作用尚不清楚。为了了解PIN1类似物在水稻中的具体作用，我们通过将我们之前创建的pin1a、pin1b和pin1c、pin1d双突变体杂交，生成了PIN1的三倍和四倍突变体。与7日龄野生型相比，pin1a pin1c pin1d和pin1b pin1c pin1d三突变体除pin1a pin1c pin1d三突变体主根和主枝较短外，无明显表型变异。pin1a、pin1b、pin1c和pin1a、pin1b、pin1d三突变体表现出主根短、根毛长、冠根和侧根少、茎短、弯曲等一系列发育异常。此外，pin1a、pin1b、pin1c、pin1d四重突变体表现出更严重的表型缺陷，是致命的。此外，在所有被检测的pin1多突变体中，一些激素信号转导和冠根发育相关基因(如OsIAAs、OsARFs、OsRRs和OsCRLs)的表达水平在茎基部都发生了显著改变。综上所述，我们的研究结果表明，四个OsPIN1同源基因在水稻生长发育调控中具有冗余功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.