Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model

سمیره نعمتی, محمدامین عدالت منش
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Abstract

Background: Prenatal Restraint stress (PRS) leads to cognitive-behavioral disorders and neuro-pathological deficits in children. The present study evaluated the effect of trichostatin A (TSA) on hippocampal level of proand anti-inflammatory cytokines in rat model of prenatal restraint stress. Materials and methods: In this experimental study, 20 pregnant Wistar rats were randomly divided into 4 groups, including control, PRS+NS, PRS+TSA5, and PRS+TSA10. PRS was induced from 13 embryonic day, one hour per day during seven days. Two hours before PRS, administration of TSA was intraperitoneally performed. At the end of one month, the hippocampal levels of pro-inflammatory cytokines such as interleukine-1β (IL-1β), interleukine-6 (IL-6),tumor necrosis factor-α (TNF-α) and anti-inflammatory cytokine (interleukine-10; IL-10) were measured by ELISA technique. Statistical differences between groups were analyzed by ANOVA and Tukey post hoc test and p˂0.05 was considered statistically significant. Results: A significant increase in pro-inflammatory cytokines content and decrease level of IL-10 in hippocampus were observed in PRS+NS group compared to the control (p˂0.05). However, TSA treatment was significantly decreased IL-1β, IL-6 and TNF-α and increased IL-10 compared to the PRS+NS group in dose manner (p˂0.05). Conclusion: It seems that TSA with anti-inflammatory effects leads to the modification of hippocampal inflammatory cytokines and reduces inflammation in offspring’s hippocampus who exposed to stress in the prenatal period.
曲古霉素A对产前应激模型大鼠海马前抗炎细胞因子的影响
背景:产前约束应激(PRS)可导致儿童认知行为障碍和神经病理缺陷。本研究探讨了曲古抑素A (trichostatin A, TSA)对产前限制性应激大鼠海马原抗炎细胞因子水平的影响。材料与方法:本实验选取妊娠Wistar大鼠20只,随机分为对照组、PRS+NS组、PRS+TSA5组和PRS+TSA10组。从胚胎第13天开始,每天1小时,共7天。PRS前2小时,腹腔注射TSA。1个月后,海马促炎细胞因子如白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)和抗炎细胞因子(白细胞介素-10;ELISA法测定白细胞介素-10 (IL-10)含量。采用方差分析(ANOVA)和Tukey事后检验(post hoc test)分析组间的统计学差异,p值小于0.05认为有统计学意义。结果:与对照组相比,PRS+NS组海马组织中促炎细胞因子含量显著升高,IL-10水平显著降低(p小于0.05)。与PRS+NS组比较,TSA治疗组IL-1β、IL-6、TNF-α显著降低,IL-10显著升高(p小于0.05)。结论:TSA具有抗炎作用,可能导致产前应激子代海马炎性细胞因子的改变,减轻了应激子代海马的炎症反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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