Anti-Toxoplasma Activity of Tellurium Oxide (TeO2) Nanoparticle on Toxoplasma gondii In vitro

Amir KarimiPourSaryazdi, F. Ghaffarifar, M. Barati, Roya Omidi
{"title":"Anti-Toxoplasma Activity of Tellurium Oxide (TeO2) Nanoparticle on Toxoplasma gondii In vitro","authors":"Amir KarimiPourSaryazdi, F. Ghaffarifar, M. Barati, Roya Omidi","doi":"10.5812/jamm.115282","DOIUrl":null,"url":null,"abstract":"Background: Toxoplasma gondii is an obligate intracellular parasite in humans and many warm-blooded animals worldwide. Objectives: This study aimed to investigate the different antiparasitic effects of tellurium oxide (TeO2) nanoparticles against T. gondii. Methods: In this study, we used tachyzoite assay (ocular counting test) to determine the lethal effect of tellurium nanoparticles on parasites. Toxic effects of nanoparticles on macrophages were also evaluated using MTT assay. The type of parasitic cell death (necrosis or apoptosis) was determined by flow cytometric analysis. Results: According to the examination of the parasites by light microscopy, TeO2 nanoparticles had destructive and lethal effects on T. gondii. The results of the MTT assay showed that the toxic effects on macrophages were more significant at high concentrations (400 μg/mL). Flow cytometric analysis also estimated the percentage of apoptosis (primary and secondary) of the parasites at 56.97%. Conclusions: According to the findings, tellurium Oxide nanoparticles have a toxic effect on T. gondii and lead to apoptosis in this parasite. This research boosts different tests in another parasite.","PeriodicalId":15058,"journal":{"name":"Journal of Archives in Military Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Archives in Military Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5812/jamm.115282","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Background: Toxoplasma gondii is an obligate intracellular parasite in humans and many warm-blooded animals worldwide. Objectives: This study aimed to investigate the different antiparasitic effects of tellurium oxide (TeO2) nanoparticles against T. gondii. Methods: In this study, we used tachyzoite assay (ocular counting test) to determine the lethal effect of tellurium nanoparticles on parasites. Toxic effects of nanoparticles on macrophages were also evaluated using MTT assay. The type of parasitic cell death (necrosis or apoptosis) was determined by flow cytometric analysis. Results: According to the examination of the parasites by light microscopy, TeO2 nanoparticles had destructive and lethal effects on T. gondii. The results of the MTT assay showed that the toxic effects on macrophages were more significant at high concentrations (400 μg/mL). Flow cytometric analysis also estimated the percentage of apoptosis (primary and secondary) of the parasites at 56.97%. Conclusions: According to the findings, tellurium Oxide nanoparticles have a toxic effect on T. gondii and lead to apoptosis in this parasite. This research boosts different tests in another parasite.
氧化碲纳米颗粒体外抗刚地弓形虫活性研究
背景:刚地弓形虫是一种专性细胞内寄生虫,存在于人类和许多温血动物体内。目的:研究氧化碲(TeO2)纳米颗粒对弓形虫的不同抗寄生作用。方法:采用速殖子法(眼计数法)测定纳米碲对寄生虫的杀伤作用。采用MTT法评价纳米颗粒对巨噬细胞的毒性作用。通过流式细胞术分析确定寄生细胞死亡类型(坏死或凋亡)。结果:光镜下观察,纳米TeO2对弓形虫具有一定的杀伤和致死作用。MTT实验结果显示,高浓度(400 μg/mL)对巨噬细胞的毒性作用更为显著。流式细胞分析还估计,寄生虫的细胞凋亡(原发性和继发性)百分比为56.97%。结论:氧化碲纳米颗粒对弓形虫具有毒性作用,可导致弓形虫细胞凋亡。这项研究促进了对另一种寄生虫的不同测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信