Mould Characterization and Mycotoxin Quantification of Chia Seeds (Salvia hispanica L.) Grown in Kenya

Veronicah Njeri, M. Mburu, K. Koskei
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引用次数: 1

Abstract

Chia seeds are functional food that have been considered highly nutritious. They have high levels of polyunsaturated fatty acid content therefore counteract lifestyle disorder such as cardiovascular diseases. This study sought to determine the level of mould contamination in chia seeds; enumeration and characterization of the types of molds and quantification of mycotoxin level, for chia seeds grown and sold in Kenya. A complete randomized block design with triplicates was used in the study. Samples were collected at random from farmers and distributors in Nyeri, Nakuru, Busia and Trans Nzoia counties. Moulds species were isolated from PDA and MEA Medias and morphological characteristics was determined under X 40 magnification power. Mould counts were found to be between 1.33 X 103cfu/ml to 2.67 X 103cfu/ml. Mould characterization done by microscopic and macroscopic technique showed evidence of Rhizopus spp, Trichoderma spp and Fusarium spp. Amongst the three genera found, Rhizopus spp was the predominantly occurring mould. The percentage moisture content of chia seeds samples ranged from 6.49±1.26 and 9.16±0.43. Significant variations on moisture content (p< 0.05) were observed among chia samples from different farmers. Aflatoxin was not detected in all chia samples. It can therefore be concluded that the chia samples were not contaminated with aflatoxin although different species of mold were present. Farmers need to be trained on proper postharvest handling methods of chia seeds, as well as proper storage and an objective method of analyzing the moisture content of the chia seeds need to be developed.
鼠尾草籽(鼠尾草)霉菌特性及霉菌毒素定量分析肯尼亚种植
奇亚籽是一种被认为营养丰富的功能性食品。它们含有高水平的多不饱和脂肪酸,因此可以抵消心血管疾病等生活方式失调。这项研究试图确定奇亚籽中霉菌污染的程度;对在肯尼亚种植和销售的奇亚籽进行霉菌种类的枚举和鉴定以及真菌毒素水平的定量分析。研究采用完全随机区组设计,有三个重复。从尼耶里、纳库鲁、布西亚和特恩佐亚县的农民和经销商中随机收集样本。分别从PDA和MEA培养基中分离霉菌种,并在x40倍率下测定其形态特征。霉菌计数在1.33 X 103cfu/ml到2.67 X 103cfu/ml之间。通过显微和宏观技术对霉菌进行了鉴定,发现有根霉属、木霉属和镰刀菌属,其中根霉属的霉菌数量最多。奇亚籽样品的水分含量范围为6.49±1.26 ~ 9.16±0.43。不同农户的奇亚样品水分含量差异显著(p< 0.05)。所有样品均未检出黄曲霉毒素。因此,尽管存在不同种类的霉菌,但chia样品未受到黄曲霉毒素的污染。需要对农民进行奇亚籽采收后处理方法的培训,以及开发适当的储存方法和分析奇亚籽含水量的客观方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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