Effect of two calcium-silicate sealers and a resin sealer on collagen matrix integrity of root dentin after different treatments. An in vitro and in vivo study
Samah Ismail, D. Fayyad, Mohamed Eldaharawy, D. Mohamed
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引用次数: 2
Abstract
Introduction: This research aimed to examine and compare the effect of two calcium silicate sealers with AH plus resin sealer on collagen extraction and surface collagen degradation of dog's root dentin, using different irrigants. Materials and Methods: In vitro part; 180 standardized root dentin cylinders obtained from dog's incisors were randomly allocated into three groups (n = 60) according to the final irrigant used; Group A: 17% Ethylenediaminetetraacetic acid, Group B: 2.5% NaOCl, Group C: 0.9% saline. Each group was classified into 4 subgroups (n = 15) according to the type of sealer used for obturation; subgroup 1: BioRoot RCS, subgroup 2: Total Fill BC, subgroup 3: AH Plus sealer, and subgroup 4: Unfilled (control). After preparation, irrigation, obturation, and aging in a storage medium; hydroxyproline (HYP) released in the medium was determined after 1 day, 1 and 3 months using spectrophotometer. In vivo part, sixty incisors of five dogs were randomly allocated as in the vitro part into three groups and four subgroups in accordance to the type of irrigation and sealer used. After 3 months of preparation, irrigation, and obturation, dogs were euthanized and teeth were extracted for the assessment of surface collagen degradation using scanning electron microscope. HYP released was analyzed using repeated-measures analysis of variance and post hoc of Bonferroni. Surface collagen degradation was analyzed using Kruskal–Wallis test and Dunn's test. Results: BioRoot RCS and TotalFill BC showed a significant higher (P < 0.001) HYP release and surface collagen degradation than AH plus sealer and control with all irrigants. Conclusions: Calcium silicate sealers significantly affected the collagen microstructure of dentin surface. AH Plus resin sealer has no effect on collagen dissolution or microstructure.
期刊介绍:
Vision SEJ aims to be one of the foremost worldwide periodical on Endodontics, dedicated to the promotion of research, post-graduate training and further education in Endodontics. Mission Statement To serve as a medium for continued Endodontic education and qualitative scientific publications on clinical trials, basic science related to the biological aspects of Endodontics, basic science related to Endodontic techniques as well as dental trauma that will ultimately improve the Endodontic research and patient’s health. Scope In this journal, Endodontists, Endodontic postgraduate students and general dentists, can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation that help them keep pace with rapid changes in this field. Aims and Objectives To publish cut edge peer-review original articles, case reports, letters to the editor, editorials, review articles, commentaries, and innovations that will impact on Endodontics. To enhance exchange of ideas/information relating to Endodontics and interaction among stakeholders. To encourage networking and partnership between individuals, government and non-governmental organizations for the provision of quality health care. To advocate for training, workshops, seminars, scientific manuscript writing conferences that will advance publishing culture.