Chukwuma S. Ezeonu , Ikechukwu N.E. Onwurah , Chibuike S. Ubani , Chigozie M. Ejikeme , Alloysius C. Ogodo
{"title":"Trichophyton Soudanense and Trichophyton Mentagrophyte-treated Rice Husk Biomass Components and Effect of Yeast on the Bioethanol Yield","authors":"Chukwuma S. Ezeonu , Ikechukwu N.E. Onwurah , Chibuike S. Ubani , Chigozie M. Ejikeme , Alloysius C. Ogodo","doi":"10.1016/j.als.2016.05.007","DOIUrl":null,"url":null,"abstract":"<div><p><em>Trichophyton soudanense</em> and <em>Trichophyton mentagrophyte</em> were isolated from the rice mill environment, cultured, and used singly and as co-culture in the treatment of measure quantities of pre-heated rice husk. Carboxymethyl cellulase activity of 57.61<!--> <!-->μg/ml/min was optimum for <em>T. mentagrophyte</em>-treated rice husk crude enzymes at 50<!--> <!-->°C and 80<!--> <!-->°C, respectively. The duration of 120<!--> <!-->h gave the highest CMCellulase activity of 75.84<!--> <!-->μg/ml/min for crude enzyme of <em>T. mentagrophyte</em> heat-pretreated rice husk. The duration of 96<!--> <!-->h gave maximum activity of 58.21<!--> <!-->μg/ml/min for crude enzyme of <em>T. soudanense</em> heat-pretreated rice husk. Highest CMCellulase activities of 67.02<!--> <!-->μg/ml/min and 69.02<!--> <!-->μg/ml/min at pH of 5 were recorded for crude enzymes of monocultures of <em>T. soudanense</em> (TS) and <em>T. mentagrophyte</em> (TM) heat-pretreated rice husk. Heating before treatment with <em>T. mentagrophyte</em> gave 44.50<!--> <!-->±<!--> <!-->10.90 cellulose as maximum, total lignin value of 28.90<!--> <!-->±<!--> <!-->1.80 from co-culture of <em>T. soudanense</em> and <em>T. mentagrophyte</em> (TS<!--> <!-->+<!--> <!-->TM), hemicellulose content of 30.50<!--> <!-->±<!--> <!-->2.12 (%<!--> <!-->±<!--> <!-->SEM) from pre-heated rice husk treated with <em>T. soudanense</em> (TS), carbohydrate content of 16.79<!--> <!-->±<!--> <!-->9.14, and reducing and non-reducing sugar values of 2.66<!--> <!-->±<!--> <!-->0.45 and 14.13<!--> <!-->±<!--> <!-->8.69 were all obtained from for pre- heated rice husk treated with <em>T. mentagrophyte</em> (TM). The pre-heated rice husk treated with <em>T. mentagrophyte (TM)</em> fermented with palm wine yeast gave bioethanol value of 11.11<!--> <!-->±<!--> <!-->0.21 (%<!--> <!-->±<!--> <!-->standard deviation) as the highest yield.</p></div>","PeriodicalId":100012,"journal":{"name":"Achievements in the Life Sciences","volume":"10 1","pages":"Pages 72-79"},"PeriodicalIF":0.0000,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.als.2016.05.007","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Achievements in the Life Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2078152015300468","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Trichophyton soudanense and Trichophyton mentagrophyte were isolated from the rice mill environment, cultured, and used singly and as co-culture in the treatment of measure quantities of pre-heated rice husk. Carboxymethyl cellulase activity of 57.61 μg/ml/min was optimum for T. mentagrophyte-treated rice husk crude enzymes at 50 °C and 80 °C, respectively. The duration of 120 h gave the highest CMCellulase activity of 75.84 μg/ml/min for crude enzyme of T. mentagrophyte heat-pretreated rice husk. The duration of 96 h gave maximum activity of 58.21 μg/ml/min for crude enzyme of T. soudanense heat-pretreated rice husk. Highest CMCellulase activities of 67.02 μg/ml/min and 69.02 μg/ml/min at pH of 5 were recorded for crude enzymes of monocultures of T. soudanense (TS) and T. mentagrophyte (TM) heat-pretreated rice husk. Heating before treatment with T. mentagrophyte gave 44.50 ± 10.90 cellulose as maximum, total lignin value of 28.90 ± 1.80 from co-culture of T. soudanense and T. mentagrophyte (TS + TM), hemicellulose content of 30.50 ± 2.12 (% ± SEM) from pre-heated rice husk treated with T. soudanense (TS), carbohydrate content of 16.79 ± 9.14, and reducing and non-reducing sugar values of 2.66 ± 0.45 and 14.13 ± 8.69 were all obtained from for pre- heated rice husk treated with T. mentagrophyte (TM). The pre-heated rice husk treated with T. mentagrophyte (TM) fermented with palm wine yeast gave bioethanol value of 11.11 ± 0.21 (% ± standard deviation) as the highest yield.
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