Characterization of a Suspension Culture of Chelidonium majus L. on Growth and Accumulation of Sanguinarine

Abstract

Cell suspension cultures of C. majus L. — obtained from callus — were characterized on growth and on accumulation of isoquinoline alkaloids. The liquid batch cultures consisted of coarse cell aggregates; their fresh volume becames triple each 14 days after subcultures. Cell cultures were found to produce relatively large amounts of isoquinoline alkaloids; sanguinarine is accumulated as main alkaloid and also released into the medium.