Inactivation and Disinfection of Porcine Parvovirus on a Nonporous Surface

Abstract

Animal parvoviruses have historically been accorded status as “highly resistant to inactivation”. This status has been based largely on the well-known heat and chemical inactivation resistance of the animal parvoviruses (especially porcine, canine, bovine, and murine parvoviruses) in liquid inactivation settings. On the other hand, less is known about the relative resistance of parvoviruses to disinfection after being dried on surfaces. In the present article, we evaluate the ability of sodium hypochlorite and two proprietary aldehyde-based disinfectants to inactivate porcine parvovirus (PPV) dried on glass carriers in the presence and absence of varying organic load. Sodium hypochlorite and Microbide-G (a glutaraldehyde-based agent) caused rapid and complete (≥ 3 to 4 log10) inactivation of PPV deposited on glass carriers in a low organic load (5% serum) matrix. Microbide-G displayed the greatest inactivation efficacy for PPV deposited onto a glass surface in a blood matrix. In that case, a contact time of 10 min resulted in 3.5 log10 inactivation at ambient temperature.