The in vitro anti-Leishmania Effect of Zingiber officinale Extract on Promastigotes and Amastigotes of Leishmania major and Leishmania tropica

IF 2.4 Q3 Computer Science

Journal of Theoretical & Computational Chemistry Pub Date : 2022-05-23 DOI:10.4274/tpd.galenos.2021.53825

Jasem Saki, Elaheh Biranvand, Reza Arjmand

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引用次数: 3

Abstract

Objective: Recently, the use of pentavalent antimony compounds for Leishmaniasis treatment has been associated with disease recurrence, drug resistance, and severe side effects. Therefore, there is a need to develop alternative treatment strategies. This study investigates the in vitro effects of Zingiber officinale on promastigotes and amastigotes of Leishmania major and Leishmania tropica.

Methods: Promastigotes and amastigotes of Leishmania major and Leishmania tropica were cultured and mass-produced in an RPMI1640 medium enriched with other necessary compounds. The MTT colorimetric method and calculating the IC50 value were used to evaluate the anti-leishmania activity of hydroalcoholic extract of Zingiber officinale.

Results: The hydroalcoholic extract of Zingiber officinale inhibited the growth of Leishmania major and Leishmania tropica promastigotes in 24, 48, and 72 hours after in vitro incubation. The IC50 of hydroalcoholic extract of Zingiber officinale was 56 μg/mL for Leishmania major and 275 μg/mL for Leishmania tropica promastigotes after 72 hours. The IC50 of hydroalcoholic extract of Zingiber officinale was 75 μg/mL for Leishmania major and 325 μg/mL for Leishmania tropica amastigotes after 72 hours.

Conclusion: The results showed that hydroalcoholic extract of Zingiber officinale has cytotoxicity properties, and Leishmania tropica has a higher resistance to hydroalcoholic extract of Zingiber officinale than Leishmania major. Further research is recommended.

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姜科植物提取物对大利什曼原虫和热带利什曼原虫的体外抗利什曼病作用

目的：最近，使用五价锑化合物治疗利什曼病与疾病复发、耐药性和严重的副作用有关。因此，有必要开发替代治疗策略。本研究调查了姜科植物对大利什曼原虫和热带利什曼原虫的体外效应：方法：在富含其他必要化合物的 RPMI1640 培养基中培养和大量繁殖大利什曼原虫和热带利什曼原虫。采用 MTT 比色法和计算 IC50 值来评价细辛水醇提取物的抗利什曼病活性：结果：在体外培养 24、48 和 72 小时后，银杏叶水醇提取物能抑制大利什曼原虫和热带利什曼原虫的生长。72 小时后，银杏叶水醇提取物对大利什曼原虫的 IC50 值为 56 μg/mL，对热带利什曼原虫的 IC50 值为 275 μg/mL。72 小时后，大利什曼原虫和热带利什曼原虫的 IC50 分别为 75 微克/毫升和 325 微克/毫升：结果表明，细辛的水醇提取物具有细胞毒性特性，而热带利什曼原虫对细辛水醇提取物的抗性高于大利什曼原虫。建议进一步开展研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Theoretical & Computational Chemistry 化学-化学综合

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Theoretical and Computational Chemistry (JTCC) is an international interdisciplinary journal aimed at providing comprehensive coverage on the latest developments and applications of research in the ever-expanding field of theoretical and computational chemistry. JTCC publishes regular articles and reviews on new methodology, software, web server and database developments. The applications of existing theoretical and computational methods which produce significant new insights into important problems are also welcomed. Papers reporting joint computational and experimental investigations are encouraged. The journal will not consider manuscripts reporting straightforward calculations of the properties of molecules with existing software packages without addressing a significant scientific problem. Areas covered by the journal include molecular dynamics, computer-aided molecular design, modeling effects of mutation on stability and dynamics of macromolecules, quantum mechanics, statistical mechanics and other related topics.