Determination of Aflatoxin Levels in Some Dairy Food Products and Dry Nuts Consumed in Saudi Arabia

Abstract

Three hundred and ninety-three samples of dairy products (124 samples of white cheese, 61 samples of cream cheese, 76 samples of Kashar cheese, and 132 samples of butter), 91 samples of cashew nut and 97 macademia nut marketed in Eastern Province, Saudi Arabia during September 2010-September 2011, were analyzed for aflatoxin M1 (AFM1), total aflatoxin and AFB1 by microtitre plate enzyme linked immunosorband assay (ELISA) The incidence of AFM1 contamination in dairy products analyzed was 82%. Total aflatoxin contamination was determined in 84 (92.3%) of 91 cashew nut and in 88 (90.7%) of 97 macademia nut whereas total aflatoxin contamination was not detected in 3 (3.2%) of 91 cashew nut samples and in 12 of 97 macademia nut samples. AFB1 was found in 84 (92.3%) of 91 cashew nut and in 83 (85.5%) of 97 samples of macademia nut samples. AFM1 levels in 3 (2.4%) white cheese, 4 (3.0%) of butter, 2 (3.2%) in cream cheese and 5 (6.5%) of Kashar cheese samples were found higher than the maximum acceptable levels as set by European Union.Continuous surveillance program may be warranted to monitor regularly the occurrence of aflatoxins in foodstuffs.