Genetic Diversity Analysis of Youxi Bitter Tea Resources Based on ISSR Molecular Markers

茶叶科学 Pub Date : 2020-01-01 DOI:10.5376/jtsr.2020.10.0003

Shasha Wei, J. Peng, Zhidan Chen, Weijiang Sun, Lin Lin, Renquan Wu

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Abstract

In this study, we analyzed the genetic diversity by using ISSR markers in four populations with 37 DNA samples of Youxi Kucha. In total, 66 alleles were amplified using 8 ISSR primers. A total of 66 bands were detected, of which 63 bands were polymorphic with a polymorphic proportion of 95.24 %. At the population level, the average PPL of the four populations of Youxi Kucha is 76.19 %, and the average values of Nei's gene diversity index (H) and Shannon diversity index (I) are 0.204 5 and 0.367 7 respectively. At the species levelm the H and I are 0.305 1 and 0.465 0 respectively respectively. Youxi Kucha maintained a relatively high genetic variability at the population level and species level bothly. The genetic differentiation within the population was significantly higher than that between populations,but the genetic differentiation between the populations has also reached higher level (Φst = 0.17> 0.15), the differentiation is extremely significant (P <0.01). A mantel test indicated there was no significant relationship between genetic distance and geographic distance among the populations studied. Correlation analysis shows that the diversity index has little relationship with altitude. This study carried out the molecular identification of the germplasm resources of Youxi bitter tea accurately, which can provide a theoretical basis for the protection of Youxi bitter tea and the breeding of improved tea varieties.

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基于ISSR分子标记的幽溪苦茶资源遗传多样性分析

本研究利用ISSR标记分析了油溪苦茶4个居群的遗传多样性。共扩增到66个等位基因，共使用8条ISSR引物。共检测到66条条带，其中多态性条带63条，多态性比例为95.24%。在种群水平上，油溪库茶4个种群的平均PPL为76.19%，Nei’s基因多样性指数(H)和Shannon多样性指数(I)的平均值分别为0.204 5和0.367 7。在种水平上，H和I分别为0.305 1和0.465 0。油溪库茶在种群水平和种水平上均保持较高的遗传变异性。居群内遗传分化显著高于居群间，居群间遗传分化也达到了较高水平(Φst = 0.17> 0.15)，分化极显著(P <0.01)。种群间遗传距离与地理距离的关系不显著。相关分析表明，多样性指数与海拔高度关系不大。本研究对幽溪苦茶种质资源进行了准确的分子鉴定，可为幽溪苦茶的保护和茶叶良种的选育提供理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

茶叶科学

CiteScore

1.50

自引率

0.00%

发文量

2558

期刊介绍： The Journal of Tea Science was established in August 1964, approved by the Publicity Department, CCCPC. Its title was inscribed by Zhu De, the chairman of CCCPC. It was discontinued during the Cultural Revolution in 1966, and it was reissued in August 1984, approved by the State Scientific and Technological Commission.Academicians Chen Zongmao and Liu Zhonghuaof the Chinese Academy of Engineering served as the directors of the editorial board. The Journal of Tea Science is managed by the China Association for Science and Technology,sponsored by the China Tea Science Society and the Tea Research Institute of the Chinese Academy of Agricultural Sciences, and edited and published by the editorial office of the Journal of Tea Science. It is the only one of Chinese core journals in the field of tea science that is included in the core library of the Chinese Science Citation Database.Its Domestic Unified Serial Number is CN 33-1115/S, its International Standard Serial Number is ISSN 1000-369X and its International publication name code is CODEN-CHKEF4. At present, the Journal of Tea Science is a bimonthly publication, published in the middle of the month, with a book size of 16.