Epigenetic changes of Arabidopsis MET1 cytosine methyltransferase mutants under salt stress

Yağmur Vecide Yeşildirek, B. Arıkan, Neslihan Turgut Kara
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引用次数: 1

Abstract

Abstract In this study, we investigated the morphological and molecular responses of Arabidopsis met1-7 and met1-3 mutants under salinity stress. Global DNA methylation changes of mutants were compared and genes known to be involved in DNA methylation in plants were analyzed. Also, the expression of the telomerase reverse transcriptase (TERT) gene as a stress response indicator was analyzed in order to examine the effects of stress on the telomerase enzyme in the mutants. We found that mutants have a higher rate of hypomethylation than Col-0, under all conditions. According to the qPCR analysis, expression levels of Domains Rearranged Methylase 2 (DRM2), Polymerase IV (Pol IV) and Polymerase V (Pol V) genes were higher in mutants than Col-0 plants. Contrary to expectations for the TERT gene, there was an increase in expression in the stress-applied mutant plants. Taken together, results suggest that the RNA-directed DNA methylation (RdDM) pathway is enhanced in mutants in order to deal with the lack of DNA methylation in CG sites. In conclusion, we believe that the morphological and molecular data obtained regarding the effects of NaCl application to met1 mutants will help us to understand the epigenetic basis of stress mechanisms.
盐胁迫下拟南芥MET1胞嘧啶甲基转移酶突变体的表观遗传变化
摘要本研究研究了拟南芥met1-7和met1-3突变体在盐胁迫下的形态和分子响应。比较了突变体的整体DNA甲基化变化,分析了已知参与植物DNA甲基化的基因。此外,我们还分析了作为应激反应指标的端粒酶逆转录酶(TERT)基因的表达,以研究应激对突变体端粒酶的影响。我们发现,在所有条件下,突变体的低甲基化率都高于Col-0。qPCR结果显示,突变体中结构域重排甲基化酶2 (DRM2)、聚合酶IV (Pol IV)和聚合酶V (Pol V)基因的表达量高于Col-0植株。与对TERT基因的预期相反,在施加胁迫的突变植物中表达增加。综上所述,结果表明,突变体中rna定向DNA甲基化(RdDM)途径增强,以解决CG位点DNA甲基化的缺乏。综上所述,我们认为NaCl处理对met1突变体影响的形态学和分子数据将有助于我们了解胁迫机制的表观遗传基础。
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