Isolation, Production, Purification, Assay and Characterization of Insulin From Goat (Capra Hircus) Pancreas By RDT Using Saccharomyces Cerevisae Vector

Abstract

Insulin is a polypeptide hormone secreted by the β-cell of Islet’s of langerhan’s of the pancreas and cosists of two polypeptide chain-A and chain- B. It is linked by two inter chain disulphide bonds (A7- B7 and A20-B19) and also has an intra-chain disulphide bond in the A-Chain (A6-A11). For production of Insulin from goat pancreas saccharomyces cerevisae using as a vector. In this study Recombinant DNA technology, chromatography, Elecrophoresis, Spectrophotometer, SDS-PAGE, zymography etc techaniques were used. Humulin was tanken as a marker. It was found, goat insulin showed good result. 5.8 – 6.5 KDa recombinant insulin was calculated. It did not show antigenic proterties.