Quantification of secondary metabolites and antimicrobial efficacy in leaves of Bruguiera gymnorrhiza – A true mangrove inwest coast of Kerala

Abstract

The present study was aimed to analyze the active compounds and quantification of secondary metabolites such as total phenol content, flavonoid content and H 2 O 2 content in the leaves of Bruguiera gymnorrhiza and carry out the antimicrobial activity of the large leafed mangrove.The ethanolic leaf extract was analyzed using GC-MS method. Quantification of secondary metabolites like total phenol, flavonoid, and H 2 O 2 content was carried out on both hexane and ethanol extracts. The antimicrobial activity was carried out on the ethanolic leaf extract against standard Ciprofloxacin. The ethanolic leaf extract of the sample showed more compromising results than that of the hexane extract. The flavonoid content and the phenol content is said to be more in the ethanol extract than that of the hexane extract. The H 2 O 2 content also came out to be more in the ethanol extract. The Gas Chromatography Mass Spectrometer of the ethanolic extract of the leaf sample revealed about 25 compounds with Formamide, n,ndimethyl showing the highest peak area. The antimicrobial activity of the leaf sample of the ethanolic extract showed more activity. The present study revealed that Bruguiera gymnorrhiza can be used as a potential drug and furthers studies are required on it.